Research Papers: Imaging

In vivo label-free quantification of liver microcirculation using dual-modality microscopy

[+] Author Affiliations
Jie Yan

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Singapore

National University of Singapore, Yong Loo Lin School of Medicine, Department of Physiology, 14 Medical Drive, MD 11 #04-01A, 117599 Singapore, Singapore

Yuzhan Kang

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Zhujiang Hospital of Southern Medical University, Department of Critical Care Medicine, 253 Gongye Middle Ave Haizhu, Guangzhou 510280,China

Shuoyu Xu

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Singapore

Lee-Ling S. Ong

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Shuangmu Zhuo

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fuzhou 350007, China

Ralph M Bunte

Duke-NUS Graduate Medical School Singapore, 8 College Road, 169857 Singapore, Singapore

Nanguang Chen

National University of Singapore, Faculty of Engineering, Department of bioengineering, 9 Engineering Drive 1,117574 Singapore, Singapore

H. Harry Asada

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Massachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States

Peter T. C. So

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Massachusetts Institute of Technology, Department of Mechanical Engineering, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States

Massachusetts Institute of Technology, Department of Biological Engineering, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States

Ian R. Wanless

Dalhousie University, Victoria General Hospital, Department of Pathology, Halifax, Nova Scotia, B3H 2Y9 Canada

Hanry Yu

BioSystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore, Singapore

Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Singapore

National University of Singapore, Yong Loo Lin School of Medicine, Department of Physiology, 14 Medical Drive, MD 11 #04-01A, 117599 Singapore, Singapore

Massachusetts Institute of Technology, Department of Biological Engineering, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States

Mechanobiology Institute, 5A Engineering Drive 1, T-Lab #05-01, 117411 Singapore, Singapore

J. Biomed. Opt. 19(11), 116006 (Nov 11, 2014). doi:10.1117/1.JBO.19.11.116006
History: Received July 18, 2014; Accepted October 21, 2014
Text Size: A A A

Abstract.  Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progression of liver disease. We have combined wide-field fluorescence microscopy and a laser speckle contrast technique to characterize hepatic microcirculation in vivo without labeling in a bile-duct ligation rat fibrosis model of biliary atresia. Through quantitative image analysis of four microcirculation parameters, we observed significant microcirculation lesion in the early to middle stages of fibrosis. This bimodal imaging method is useful to assess hepatic microcirculation lesion for the study of liver diseases.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Topics

Liver ; Microscopy ; Tissues

Citation

Jie Yan ; Yuzhan Kang ; Shuoyu Xu ; Lee-Ling S. Ong ; Shuangmu Zhuo, et al.
"In vivo label-free quantification of liver microcirculation using dual-modality microscopy", J. Biomed. Opt. 19(11), 116006 (Nov 11, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.11.116006


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.