Research Papers: Sensing

Delayed luminescence to monitor programmed cell death induced by berberine on thyroid cancer cells

[+] Author Affiliations
Agata Scordino

University of Catania, Department of Physics and Astronomy, via Santa Sofia 64, Catania I95123, Italy

Southern National Laboratories of National Institute for Nuclear Physics, via Santa Sofia 62, Catania I95123, Italy

Agata Campisi

University of Catania, Department of Drugs Science, viale Andrea Doria 6, Catania I95125, Italy

Rosaria Grasso

Southern National Laboratories of National Institute for Nuclear Physics, via Santa Sofia 62, Catania I95123, Italy

Roberta Bonfanti

University of Catania, Department of Drugs Science, viale Andrea Doria 6, Catania I95125, Italy

Marisa Gulino

Southern National Laboratories of National Institute for Nuclear Physics, via Santa Sofia 62, Catania I95123, Italy

“Kore” University, Faculty of Engineering, Architecture and Physical Education, Via delle Olimpiadi, Enna I94100, Italy

Liliana Iauk

University of Catania, Department of Bio-Medical Science, viale Andrea Doria 6, Catania I95125, Italy

Rosalba Parenti

University of Catania, Department of Bio-Medical Science, viale Andrea Doria 6, Catania I95125, Italy

Francesco Musumeci

University of Catania, Department of Physics and Astronomy, via Santa Sofia 64, Catania I95123, Italy

Southern National Laboratories of National Institute for Nuclear Physics, via Santa Sofia 62, Catania I95123, Italy

J. Biomed. Opt. 19(11), 117005 (Nov 13, 2014). doi:10.1117/1.JBO.19.11.117005
History: Received July 31, 2014; Accepted October 17, 2014
Text Size: A A A

Abstract.  Correlation between apoptosis and UVA-induced ultraweak photon emission delayed luminescence (DL) from tumor thyroid cell lines was investigated. In particular, the effects of berberine, an alkaloid that has been reported to have anticancer activities, on two cancer cell lines were studied. The FTC-133 and 8305C cell lines, as representative of follicular and anaplastic thyroid human cancer, respectively, were chosen. The results show that berberine is able to arrest cell cycle and activate apoptotic pathway as shown in both cell lines by deoxyribonucleic acid fragmentation, caspase-3 cleavage, p53 and p27 protein overexpression. In parallel, changes in DL spectral components after berberine treatment support the hypothesis that DL from human cells originates mainly from mitochondria, since berberine acts especially at the mitochondrial level. The decrease of DL blue component for both cell lines could be related to the decrease of intra-mitochondrial nicotinamide adenine dinucleotide and may be a hallmark of induced apoptosis. In contrast, the response in the red spectral range is different for the two cell lines and may be ascribed to a different iron homeostasis.

Figures in this Article
© 2014 Society of Photo-Optical Instrumentation Engineers

Topics

Cancer ; Luminescence

Citation

Agata Scordino ; Agata Campisi ; Rosaria Grasso ; Roberta Bonfanti ; Marisa Gulino, et al.
"Delayed luminescence to monitor programmed cell death induced by berberine on thyroid cancer cells", J. Biomed. Opt. 19(11), 117005 (Nov 13, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.11.117005


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.