Special Section on Laser Applications in Life Sciences

Penetration of silver nanoparticles into porcine skin ex vivo using fluorescence lifetime imaging microscopy, Raman microscopy, and surface-enhanced Raman scattering microscopy

[+] Author Affiliations
Yongjian Zhu

Charité–Universitätsmedizin Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitéplatz 1, Berlin 10117, Germany

Zhejiang University of Science and Technology, Department of Mechanical and Automotive Engineering, Hangzhou 310023, China

Chun-Sik Choe

Charité–Universitätsmedizin Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitéplatz 1, Berlin 10117, Germany

Kim Il Sung University, Department of Physics, Ryongnam-Dong, Taesong District, Pyongyang, Democratic People’s Republic of Korea

Sebastian Ahlberg

Charité–Universitätsmedizin Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitéplatz 1, Berlin 10117, Germany

Martina C. Meinke

Charité–Universitätsmedizin Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitéplatz 1, Berlin 10117, Germany

Ulrike Alexiev

Freie Universität Berlin, Department of Physics, Arnimallee 14, 14195 Berlin, Germany

Juergen Lademann

Charité–Universitätsmedizin Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitéplatz 1, Berlin 10117, Germany

Maxim E. Darvin

Charité–Universitätsmedizin Berlin, Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charitéplatz 1, Berlin 10117, Germany

J. Biomed. Opt. 20(5), 051006 (Nov 13, 2014). doi:10.1117/1.JBO.20.5.051006
History: Received August 26, 2014; Accepted October 21, 2014
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Abstract.  In order to investigate the penetration depth of silver nanoparticles (Ag NPs) inside the skin, porcine ears treated with Ag NPs are measured by two-photon tomography with a fluorescence lifetime imaging microscopy (TPT-FLIM) technique, confocal Raman microscopy (CRM), and surface-enhanced Raman scattering (SERS) microscopy. Ag NPs are coated with poly-N-vinylpyrrolidone and dispersed in pure water solutions. After the application of Ag NPs, porcine ears are stored in the incubator for 24 h at a temperature of 37°C. The TPT-FLIM measurement results show a dramatic decrease of the Ag NPs’ signal intensity from the skin surface to a depth of 4μm. Below 4μm, the Ag NPs’ signal continues to decline, having completely disappeared at 12 to 14μm depth. CRM shows that the penetration depth of Ag NPs is 11.1±2.1μm. The penetration depth measured with a highly sensitive SERS microscopy reaches 15.6±8.3μm. Several results obtained with SERS show that the penetration depth of Ag NPs can exceed the stratum corneum (SC) thickness, which can be explained by both penetration of trace amounts of Ag NPs through the SC barrier and by the measurements inside the hair follicle, which cannot be excluded in the experiment.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Yongjian Zhu ; Chun-Sik Choe ; Sebastian Ahlberg ; Martina C. Meinke ; Ulrike Alexiev, et al.
"Penetration of silver nanoparticles into porcine skin ex vivo using fluorescence lifetime imaging microscopy, Raman microscopy, and surface-enhanced Raman scattering microscopy", J. Biomed. Opt. 20(5), 051006 (Nov 13, 2014). ; http://dx.doi.org/10.1117/1.JBO.20.5.051006


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