Research Papers: Imaging

Depth-enhanced fluorescence imaging using masked detection of structured illumination

[+] Author Affiliations
Joseph Angelo

Beth Israel Deaconess Medical Center, Department of Medicine, 330 Brookline Avenue, Boston, Massachusetts 02215, United States

Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, United States

Vivek Venugopal, Sylvain Gioux

Beth Israel Deaconess Medical Center, Department of Medicine, 330 Brookline Avenue, Boston, Massachusetts 02215, United States

Frederic Fantoni, Vincent Poher, Lionel Herve, Jean-Marc Dinten

CEA-LETI, Minatec Campus, Grenoble 38054, France

Irving J. Bigio

Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, United States

Boston University, Department of Electrical & Computer Engineering, Boston, Massachusetts 02215, United States

J. Biomed. Opt. 19(11), 116008 (Nov 14, 2014). doi:10.1117/1.JBO.19.11.116008
History: Received September 9, 2014; Revised 00 00, 0000; Accepted October 16, 2014
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Abstract.  There is a growing interest in imaging fluorescence contrast at depth within living tissues over wide fields of view and in real time. Most methods used to date to improve depth detection of fluorescence information involve acquisition of multiple images, postprocessing of the data using a light propagation model, and are capable of providing either depth-sectioned or tomographic fluorescence information. We introduce a method, termed masked detection of structured illumination, that allows the enhancement of fluorescence imaging at depth without postprocessing. This method relies on the scanning of a collimated beam onto a turbid medium and the physical masking of the point spread function on the detection arm before acquisition on a CCD camera. By preferentially collecting diffuse photons at a chosen source-detector range, this method enhances fluorescence information at depth and has the potential to form images without postprocessing and in real time.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Joseph Angelo ; Vivek Venugopal ; Frederic Fantoni ; Vincent Poher ; Irving J. Bigio, et al.
"Depth-enhanced fluorescence imaging using masked detection of structured illumination", J. Biomed. Opt. 19(11), 116008 (Nov 14, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.11.116008


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