RP is the frequently selected treatment option for men with localized prostate cancer. However, a significant challenge faced by surgeons during RP is the complete removal of the cancerous tissue, while preserving the nerves surrounding the prostate that are responsible for continence and erectile function. These nerves, as well as the malignant glands, are too small to be visualized by the human eye. Although RP with the da Vinci robotic surgical system allows for a significant advantage in surgical precision due to 10 to magnification of the surgical field, it lacks the cellular resolution to differentiate cancerous cells from surrounding nerve tissue. Thus, some surgeons rely on intraoperative frozen section (IFS) analysis to provide some benefit in reducing positive surgical margins (PSMs).5 However, frozen sections require time and only provide an assessment of a fraction of the area of interest. Further, as IFS requires the removal of tissue, there is still a risk of damaging the area one is trying to preserve, especially the periprostatic nerves. RPs have PSMs6 in 13.8% to 22.8% of cases and a 6% to 27% rate of postoperative impotence.7 In essence, inaccurate disease quantification, staging, and unavailability of intraoperative pathological guidance often result in mismatched treatment recommendations, overtreatment, residual cancer (positive surgical margins) during surgery, and the need for expensive radiation treatment to salvage these cancers. Thus, for both the diagnosis and the treatment of prostate cancer, a faster and more accurate way of characterizing the tissue at a cellular level could significantly improve decision making during treatment and patient outcomes.