Special Section on Laser Applications in Life Sciences

Characterization of atopic skin and the effect of a hyperforin-rich cream by laser scanning microscopy

[+] Author Affiliations
Martina C. Meinke

Charité-Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin 10117, Germany

Heike Richter

Charité-Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin 10117, Germany

Anke Kleemann

Klosterfrau Berlin GmbH, Berlin 12277, Germany

Juergen Lademann

Charité-Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin 10117, Germany

Kathrin Tscherch

University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Hamburg 20146, Germany

Sascha Rohn

University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Hamburg 20146, Germany

Christoph M. Schempp

University Medical Center Freiburg, Research Center Skintegral, Freiburg 79104, Germany

J. Biomed. Opt. 20(5), 051013 (Dec 02, 2014). doi:10.1117/1.JBO.20.5.051013
History: Received August 20, 2014; Accepted October 30, 2014
Text Size: A A A

Abstract.  Atopic dermatitis (AD) is a multifactorial inflammatory skin disease that affects both children and adults in an increasing manner. The treatment of AD often reduces subjective skin parameters, such as itching, dryness, and tension, but the inflammation cannot be cured. Laser scanning microscopy was used to investigate the skin surface, epidermal, and dermal characteristics of dry and atopic skin before and after treatment with an ointment rich in hyperforin, which is known for its anti-inflammatory effects. The results were compared to subjective parameters and transepidermal water loss, stratum corneum moisture, and stratum corneum lipids. Using biophysical methods, in particular laser scanning microscopy, it was found that atopic skin has distinct features compared to healthy skin. Treatment with a hyperforin-rich ointment resulted in an improvement of the stratum corneum moisture, skin surface dryness, skin lipids, and the subjective skin parameters, indicating that the barrier is stabilized and improved by the ointment. But in contrast to the improved skin surface, the inflammation in the deeper epidermis/dermis often continues to exist. This could be clearly shown by the reflectance confocal microscopy (RCM) measurements. Therefore, RCM measurements could be used to investigate the progress in treatment of atopic dermatitis.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Martina C. Meinke ; Heike Richter ; Anke Kleemann ; Juergen Lademann ; Kathrin Tscherch, et al.
"Characterization of atopic skin and the effect of a hyperforin-rich cream by laser scanning microscopy", J. Biomed. Opt. 20(5), 051013 (Dec 02, 2014). ; http://dx.doi.org/10.1117/1.JBO.20.5.051013


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.