Research Papers: Imaging

Gaussian beam in two-photon fluorescence imaging of rat brain microvessel

[+] Author Affiliations
Lingyan Shi

The City College of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031, United States

The City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United States

The City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Convent Avenue, New York, New York 10031, United States

Adrián Rodríguez-Contreras

The City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United States

The City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Convent Avenue, New York, New York 10031, United States

Robert R. Alfano

The City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Convent Avenue, New York, New York 10031, United States

J. Biomed. Opt. 19(12), 126006 (Dec 09, 2014). doi:10.1117/1.JBO.19.12.126006
History: Received September 25, 2014; Accepted November 5, 2014
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Abstract.  The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Lingyan Shi ; Adrián Rodríguez-Contreras and Robert R. Alfano
"Gaussian beam in two-photon fluorescence imaging of rat brain microvessel", J. Biomed. Opt. 19(12), 126006 (Dec 09, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.12.126006


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