Research Papers: General

Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases

[+] Author Affiliations
Laura A. Sordillo

The City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031, United States

Peter P. Sordillo

The City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031, United States

Yury Budansky

The City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031, United States

Yang Pu

The City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031, United States

Robert R. Alfano

The City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, 160 Convent Avenue, New York, New York 10031, United States

J. Biomed. Opt. 19(12), 125002 (Dec 18, 2014). doi:10.1117/1.JBO.19.12.125002
History: Received September 23, 2014; Accepted November 24, 2014
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Abstract.  The correlation between histologic grade, an increasingly important measure of prognosis for patients with breast cancer, and tryptophan levels from tissues of 15 breast carcinoma patients was investigated. Changes in the relative content of key native organic biomolecule tryptophan were seen from the fluorescence spectra of cancerous and paired normal tissues with excitation wavelengths of 280 and 300 nm. Due to a large spectral overlap and matching excitation–emission spectra, fluorescence resonance energy transfer from tryptophan-donor to reduced nicotinamide adenine dinucleotides-acceptor was noted. We used the ratios of fluorescence intensities at their spectral emission peaks, or spectral fingerprint peaks, at 340, 440, and 460 nm. Higher ratios correlated strongly with high histologic grade, while lower-grade tumors had low ratios. Large tumor size also correlated with high ratios, while the number of lymph node metastases, a major factor in staging, was not correlated with tryptophan levels. High histologic grade correlates strongly with increased content of tryptophan in breast cancer tissues and suggests that measurement of tryptophan content may be useful as a part of the evaluation of these patients.

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© 2014 Society of Photo-Optical Instrumentation Engineers

Citation

Laura A. Sordillo ; Peter P. Sordillo ; Yury Budansky ; Yang Pu and Robert R. Alfano
"Differences in fluorescence profiles from breast cancer tissues due to changes in relative tryptophan content via energy transfer: tryptophan content correlates with histologic grade and tumor size but not with lymph node metastases", J. Biomed. Opt. 19(12), 125002 (Dec 18, 2014). ; http://dx.doi.org/10.1117/1.JBO.19.12.125002


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