Special Section on Laser Applications in Life Sciences

Diffusion characteristics of ethylene glycol in skeletal muscle

[+] Author Affiliations
Luís M. Oliveira

Polytechnic of Porto, School of Engineering, Physics Department, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal

Porto University, School of Engineering, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal

Centre of Innovation in Engineering and Industrial Technology, ISEP, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal

Maria Inês Carvalho

Porto University, School of Engineering, DEEC, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal

INESC TEC, FEUP, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal

Elisabete M. Nogueira

Polytechnic of Porto, School of Engineering, Physics Department, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal

Centre of Innovation in Engineering and Industrial Technology, ISEP, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal

Valery V. Tuchin

Saratov State University, Research-Educational Institute of Optics and Biophotonics, 83, Astrakhanskaya Street, Saratov 410012, Russia

Institute of Precise Mechanics and Control RAS, Laboratory of Laser Diagnostics of Technical and Living Systems, 24 Rabochaya Street, Saratov 410028, Russia

University of Oulu, Optoelectronics and Measurement Techniques Laboratory, PO Box 4500, 90014 Oulu, Finland

J. Biomed. Opt. 20(5), 051019 (Dec 19, 2014). doi:10.1117/1.JBO.20.5.051019
History: Received July 24, 2014; Accepted November 21, 2014
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Abstract.  Part of the optical clearing study in biological tissues concerns the determination of the diffusion characteristics of water and optical clearing agents in the subject tissue. Such information is sufficient to characterize the time dependence of the optical clearing mechanisms—tissue dehydration and refractive index (RI) matching. We have used a simple method based on collimated optical transmittance measurements made from muscle samples under treatment with aqueous solutions containing different concentrations of ethylene glycol (EG), to determine the diffusion time values of water and EG in skeletal muscle. By representing the estimated mean diffusion time values from each treatment as a function of agent concentration in solution, we could identify the real diffusion times for water and agent. These values allowed for the calculation of the correspondent diffusion coefficients for those fluids. With these results, we have demonstrated that the dehydration mechanism is the one that dominates optical clearing in the first minute of treatment, while the RI matching takes over the optical clearing operations after that and remains for a longer time of treatment up to about 10 min, as we could see for EG and thin tissue samples of 0.5 mm.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Luís M. Oliveira ; Maria Inês Carvalho ; Elisabete M. Nogueira and Valery V. Tuchin
"Diffusion characteristics of ethylene glycol in skeletal muscle", J. Biomed. Opt. 20(5), 051019 (Dec 19, 2014). ; http://dx.doi.org/10.1117/1.JBO.20.5.051019


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