Special Section on Laser Applications in Life Sciences

Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing

[+] Author Affiliations
Ryan Spitler

University of California Irvine, Irvine, California 92612 United States

Hsiang Ho, Frederique Norpetlian, Xiangduo Kong, Kyoko Yokomori, Bogi Andersen

University of California Irvine, Irvine, California 92612 United States

Jingjing Jiang, Gerry R. Boss

University of California San Diego, La Jolla, California 92093 United States

Michael W. Berns

University of California Irvine, Irvine, California 92612 United States

University of California San Diego, La Jolla, California 92093 United States

J. Biomed. Opt. 20(5), 051022 (Jan 06, 2015). doi:10.1117/1.JBO.20.5.051022
History: Received July 1, 2014; Accepted December 4, 2014
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Abstract.  Low level light therapy (LLLT) has numerous therapeutic benefits, including improving wound healing, but the precise mechanisms involved are not well established; in particular, the underlying role of cytochrome C oxidase (C-ox) as the primary photoacceptor and the associated biochemical mechanisms still require further investigation. We previously showed the nitric oxide (NO) donating drug nitrosyl-cobinamide (NO-Cbi) enhances wound healing through a cGMP/cGMP-dependent protein kinase/ERK1/2 mechanism. Here, we show that the combination of LLLT and NO-Cbi markedly improves wound healing compared to either treatment alone. LLLT-enhanced wound healing proceeded through an electron transport chain-C-ox-dependent mechanism with a reduction of reactive oxygen species and increased adenosine triphosphate production. C-ox was validated as the primary photoacceptor by three observations: increased oxygen consumption, reduced wound healing in the presence of sodium azide, and disassociation of cyanide, a known C-ox ligand, following LLLT. We conclude that LLLT and NO-Cbi accelerate wound healing through two independent mechanisms, the electron transport chain-C-ox pathway and cGMP signaling, respectively, with both resulting in ERK1/2 activation.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Ryan Spitler ; Hsiang Ho ; Frederique Norpetlian ; Xiangduo Kong ; Jingjing Jiang, et al.
"Combination of low level light therapy and nitrosyl-cobinamide accelerates wound healing", J. Biomed. Opt. 20(5), 051022 (Jan 06, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051022


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