Special Section on Laser Applications in Life Sciences

Optical coherence tomography guided microinjections in live mouse embryos: high-resolution targeted manipulation for mouse embryonic research

[+] Author Affiliations
Saba H. Syed

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030, United States

Andrew J. Coughlin

Duke University, Department of Biomedical Engineering, Hudson Hall, Durham, North Carolina 27708, United States

Monica D. Garcia

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030, United States

Shang Wang

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030, United States

Jennifer L. West

Duke University, Department of Biomedical Engineering, Hudson Hall, Durham, North Carolina 27708, United States

Kirill V. Larin

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030, United States

University of Houston, Department of Biomedical Engineering, 4605 Cullen Boulevard, Houston, Texas 77204, United States

Irina V. Larina

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030, United States

J. Biomed. Opt. 20(5), 051020 (Jan 12, 2015). doi:10.1117/1.JBO.20.5.051020
History: Received September 29, 2014; Accepted December 2, 2014
Text Size: A A A

Abstract.  The ability to conduct highly localized delivery of contrast agents, viral vectors, therapeutic or pharmacological agents, and signaling molecules or dyes to live mammalian embryos is greatly desired to enable a variety of studies in the field of developmental biology, such as investigating the molecular regulation of cardiovascular morphogenesis. To meet such a demand, we introduce, for the first time, the concept of employing optical coherence tomography (OCT)-guide microinjections in live mouse embryos, which provides precisely targeted manipulation with spatial resolution at the micrometer scale. The feasibility demonstration is performed with experimental studies on cultured live mouse embryos at E8.5 and E9.5. Additionally, we investigate the OCT-guided microinjection of gold–silica nanoshells to the yolk sac vasculature of live cultured mouse embryos at the stage when the heart just starts to beat, as a potential approach for dynamic assessment of cardiovascular form and function before the onset of blood cell circulation. Also, the capability of OCT to quantitatively monitor and measure injection volume is presented. Our results indicate that OCT-guided microinjection could be a useful tool for mouse embryonic research.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Saba H. Syed ; Andrew J. Coughlin ; Monica D. Garcia ; Shang Wang ; Jennifer L. West, et al.
"Optical coherence tomography guided microinjections in live mouse embryos: high-resolution targeted manipulation for mouse embryonic research", J. Biomed. Opt. 20(5), 051020 (Jan 12, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051020


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.