Research Papers: Imaging

Optical coherence tomography based microangiography for quantitative monitoring of structural and vascular changes in a rat model of acute uveitis in vivo: a preliminary study

[+] Author Affiliations
Woo June Choi, Zhongwei Zhi

University of Washington, Department of Bioengineering, Seattle 98195, Washington, United States

Kathryn L. Pepple

University of Washington, Department of Ophthalmology, Seattle 98104, Washington, United States

Ruikang K. Wang

University of Washington, Department of Bioengineering, Seattle 98195, Washington, United States

University of Washington, Department of Ophthalmology, Seattle 98104, Washington, United States

J. Biomed. Opt. 20(1), 016015 (Jan 16, 2015). doi:10.1117/1.JBO.20.1.016015
History: Received November 6, 2014; Accepted December 19, 2014
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Abstract.  Uveitis models in rodents are important in the investigation of pathogenesis in human uveitis and the development of appropriate therapeutic strategies for treatment. Quantitative monitoring of ocular inflammation in small animal models provides an objective metric to assess uveitis progression and/or therapeutic effects. We present a new application of optical coherence tomography (OCT) and OCT-based microangiography (OMAG) to a rat model of acute anterior uveitis induced by intravitreal injection of a killed mycobacterial extract. OCT/OMAG is used to provide noninvasive three-dimensional imaging of the anterior segment of the eyes prior to injection (baseline) and two days post-injection (peak inflammation) in rats with and without steroid treatments. OCT imaging identifies characteristic structural and vascular changes in the anterior segment of the inflamed animals when compared to baseline images. Characteristics of inflammation identified include anterior chamber cells, corneal edema, pupillary membranes, and iris vasodilation. In contrast, no significant difference from the control is observed for the steroid-treated eye. These findings are compared with the histology assessment of the same eyes. In addition, quantitative measurements of central corneal thickness and iris vessel diameter are determined. This pilot study demonstrates that OCT-based microangiography promises to be a useful tool for the assessment and management of uveitis in vivo.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Woo June Choi ; Kathryn L. Pepple ; Zhongwei Zhi and Ruikang K. Wang
"Optical coherence tomography based microangiography for quantitative monitoring of structural and vascular changes in a rat model of acute uveitis in vivo: a preliminary study", J. Biomed. Opt. 20(1), 016015 (Jan 16, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.1.016015


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