Research Papers: Imaging

Visualizing and quantifying difference in cytoplasmic and nuclear metabolism in the hepatobiliary system in vivo

[+] Author Affiliations
Chih-Ju Lin, Ning Kang, Jian-Ye Lee

National Taiwan University, Department of Physics, Taipei 106, Taiwan

Hsuan-Shu Lee

National Taiwan University Hospital and National Taiwan University, College of Medicine, Department of Internal Medicine, Taipei 100, Taiwan

National Taiwan University, Institute of Biotechnology, Taipei 106, Taiwan

Chen-Yuan Dong

National Taiwan University, Department of Physics, Taipei 106, Taiwan

National Taiwan University, Center for Optoelectronic Biomedicine, Taipei 106, Taiwan

National Taiwan University, Center for Quantum Science and Engineering, Taipei 106, Taiwan

J. Biomed. Opt. 20(1), 016020 (Jan 22, 2015). doi:10.1117/1.JBO.20.1.016020
History: Received October 30, 2014; Accepted December 30, 2014
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Abstract.  The liver is a major organ responsible for performing xenobiotic metabolism. In this process, xenobiotic is uptaken and processed in hepatocytes and subsequently excreted into the bile canaliculi. However, the intracellular heterogeneity in such metabolic processes is not known. We use the molecular probe 6-carboxyfluorescein diacetate (6-CFDA) to investigate xenobiotic metabolism in hepatocytes with intravital multiphoton fluorescence microscopy. 6-CFDA is processed by intracellular esterase to fluorescent 6-CF, which can be imaged and quantified. We found that compared to the nucleus, cytoplasmic 6-CF fluorescence intensity reached a maximum earlier (cytoplasm: 11.3±4.4min; nucleus: 14.7±4.9min) following 6-CFDA injection. We also found a slight difference in the rate of 6-CFDA metabolism as the rates of 6-CF decay at rates of 1.43±0.75 and 1.27±0.72photons/min for the cytoplasm and nucleus, respectively. These results indicate that molecular transport to the nucleus is additionally hindered and can affect drug transport there.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Chih-Ju Lin ; Ning Kang ; Jian-Ye Lee ; Hsuan-Shu Lee and Chen-Yuan Dong
"Visualizing and quantifying difference in cytoplasmic and nuclear metabolism in the hepatobiliary system in vivo", J. Biomed. Opt. 20(1), 016020 (Jan 22, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.1.016020


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