Special Section on Laser Applications in Life Sciences

Photobleaching effects on in vivo skin autofluorescence lifetime

[+] Author Affiliations
Inesa Ferulova

University of Latvia, 19 Raina Boulevard, Riga 1586, Latvia

Alexey Lihachev

University of Latvia, 19 Raina Boulevard, Riga 1586, Latvia

Janis Spigulis

University of Latvia, 19 Raina Boulevard, Riga 1586, Latvia

J. Biomed. Opt. 20(5), 051031 (Jan 30, 2015). doi:10.1117/1.JBO.20.5.051031
History: Received September 1, 2014; Accepted January 7, 2015
Text Size: A A A

Abstract.  The autofluorescence lifetime of healthy human skin was measured using excitation provided by a picosecond diode laser operating at a wavelength of 405 nm and with fluorescence emission collected at 475 and 560 nm. In addition, spectral and temporal responses of healthy human skin and intradermal nevus in the spectral range 460 to 610 nm were studied before and after photobleaching. A decrease in the autofluorescences lifetimes changes was observed after photobleaching of human skin. A three-exponential model was used to fit the signals, and under this model, the most significant photoinduced changes were observed for the slowest lifetime component in healthy skin at the spectral range 520 to 610 nm and intradermal nevus at the spectral range 460 to 610 nm.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Topics

Skin ; Luminescence

Citation

Inesa Ferulova ; Alexey Lihachev and Janis Spigulis
"Photobleaching effects on in vivo skin autofluorescence lifetime", J. Biomed. Opt. 20(5), 051031 (Jan 30, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051031


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
DEEP, SHALLOW AND EYE LENS DOSES FROM 106RU/106RH-A COMPARSION. Radiat Prot Dosimetry Published online Jan 23, 2017;
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.