Research Papers: General

Kinetics of red blood cell rouleaux formation studied by light scattering

[+] Author Affiliations
Alicja Szołna-Chodór

Nicolaus Copernicus University, Biophysics Department, Collegium Medicum in Bydgoszcz, ul. Jagiellońska 15, Bydgoszcz 85-067, Poland

Maciej Bosek

Nicolaus Copernicus University, Biophysics Department, Collegium Medicum in Bydgoszcz, ul. Jagiellońska 15, Bydgoszcz 85-067, Poland

Bronisław Grzegorzewski

Nicolaus Copernicus University, Biophysics Department, Collegium Medicum in Bydgoszcz, ul. Jagiellońska 15, Bydgoszcz 85-067, Poland

J. Biomed. Opt. 20(2), 025001 (Feb 03, 2015). doi:10.1117/1.JBO.20.2.025001
History: Received September 18, 2014; Accepted January 9, 2015
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Abstract.  Red blood cell (RBC) rouleaux formation was experimentally studied using a light scattering technique. The suspensions of RBCs were obtained from the blood of healthy donors. Hematocrit of the samples was adjusted ranging from 1% to 4%. Measurements of the intensity of the coherent component of light scattered by the suspensions were performed and the scattering coefficient of the suspensions was determined. The number of RBCs per rouleaux was obtained using anomalous diffraction theory. The technique was used to show the effect of time, hematocrit, and sample thickness on the process. The number of cells per rouleaux first increases linearly, reaches a critical value at 3 cells per rouleaux, and then a further increase in the rouleaux size is observed. The kinetic constant of the rouleaux growth in the linear region is found to be independent of hematocrit. The aggregation rate increases as the sample thickness increases. The time at which the critical region appears strongly decreases as the hematocrit of the suspension increases.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Alicja Szołna-Chodór ; Maciej Bosek and Bronisław Grzegorzewski
"Kinetics of red blood cell rouleaux formation studied by light scattering", J. Biomed. Opt. 20(2), 025001 (Feb 03, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.2.025001


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