Research Papers: Imaging

Macroscopic optical imaging technique for wide-field estimation of fluorescence depth in optically turbid media for application in brain tumor surgical guidance

[+] Author Affiliations
Kolbein K. Kolste, Stephen C. Kanick, Keith D. Paulsen

Dartmouth College, Thayer School of Engineering, Hanover, 14 Engineering Drive, New Hampshire 03755, United States

Pablo A. Valdés

Dartmouth College, Thayer School of Engineering, Hanover, 14 Engineering Drive, New Hampshire 03755, United States

Dartmouth College, Geisel School of Medicine, Hanover, 1 Rope Ferry Road, New Hampshire 03755, United States

Michael Jermyn, Frederic Leblond

Polytechnique Montreal, Engineering Physics Department, Montreal, Québec H3C 3A7, Canada

Brian C. Wilson

University of Toronto, Ontario Cancer Institute, 610 University Avenue, Toronto, Ontario M5G 2M9, Canada

David W. Roberts

Dartmouth-Hitchcock Medical Center, Section of Neurosurgery, 1 Medical Center Drive, Lebanon, New Hampshire 03756, United States

J. Biomed. Opt. 20(2), 026002 (Feb 04, 2015). doi:10.1117/1.JBO.20.2.026002
History: Received October 8, 2014; Accepted January 5, 2015
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Abstract.  A diffuse imaging method is presented that enables wide-field estimation of the depth of fluorescent molecular markers in turbid media by quantifying the deformation of the detected fluorescence spectra due to the wavelength-dependent light attenuation by overlying tissue. This is achieved by measuring the ratio of the fluorescence at two wavelengths in combination with normalization techniques based on diffuse reflectance measurements to evaluate tissue attenuation variations for different depths. It is demonstrated that fluorescence topography can be achieved up to a 5 mm depth using a near-infrared dye with millimeter depth accuracy in turbid media having optical properties representative of normal brain tissue. Wide-field depth estimates are made using optical technology integrated onto a commercial surgical microscope, making this approach feasible for real-world applications.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Kolbein K. Kolste ; Stephen C. Kanick ; Pablo A. Valdés ; Michael Jermyn ; Brian C. Wilson, et al.
"Macroscopic optical imaging technique for wide-field estimation of fluorescence depth in optically turbid media for application in brain tumor surgical guidance", J. Biomed. Opt. 20(2), 026002 (Feb 04, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.2.026002


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