Special Section on Laser Applications in Life Sciences

Multidimensional visualization of healthy and sensitized rabbit knee tissues by means of confocal microscopy

[+] Author Affiliations
Romualdas Rudys

The National Cancer Institute, P. Baublio 3b, Vilnius LT 08406, Lithuania

State Research Institute, Center for Innovative Medicine, Žygimantų 9, Vilnius LT 01102, Lithuania

Saulius Bagdonas

Vilnius University, Biophotonics Group of Laser Research Center, Faculty of Physics, Saulėtekio 9, Building 3, Vilnius LT-10222, Lithuania

Gailutė Kirdaitė, Jurga Papečkienė

State Research Institute, Center for Innovative Medicine, Žygimantų 9, Vilnius LT 01102, Lithuania

Ričardas Rotomskis

The National Cancer Institute, P. Baublio 3b, Vilnius LT 08406, Lithuania

Vilnius University, Biophotonics Group of Laser Research Center, Faculty of Physics, Saulėtekio 9, Building 3, Vilnius LT-10222, Lithuania

J. Biomed. Opt. 20(5), 051035 (Feb 11, 2015). doi:10.1117/1.JBO.20.5.051035
History: Received August 31, 2014; Accepted January 19, 2015
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Abstract.  This study combines several fluorescence detection methods to distinguish structural features of the synovium and cartilage tissues and to visualize the localization of endogenous porphyrins in the sensitized tissues. Specimens of synovium and cartilage tissues obtained from rabbits with antigen-induced monoarthritis after intra-articular 5-aminolevulinic acid methyl ester injection and those from healthy rabbits were investigated ex vivo by means of fluorescence spectroscopy, fluorescence intensity, and lifetime microscopy. The presence of endogenous porphyrins was confirmed with the fluorescence spectra measured on sliced sensitized specimens. Application of the lifetime-gating method on fast fluorescence lifetime imaging microscopy images, allowed separate visualization of tissue structures possessing different average lifetimes. The presence of the structures has been validated by histopathological imaging based on conventional rapid hematoxylin–eosin staining of the specimens. The fluorescence lifetime of endogenous protoporphyrin IX has been assessed and employed for visualization of sensitized tissues.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Romualdas Rudys ; Saulius Bagdonas ; Gailutė Kirdaitė ; Jurga Papečkienė and Ričardas Rotomskis
"Multidimensional visualization of healthy and sensitized rabbit knee tissues by means of confocal microscopy", J. Biomed. Opt. 20(5), 051035 (Feb 11, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051035


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