Special Section on Laser Applications in Life Sciences

Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes: identifying fingerprints of glucose-related metabolic pathways

[+] Author Affiliations
Evgeny Shirshin, Elena Berlovskaya, Victor Fadeev

M.V. Lomonosov Moscow State University, Physical Department, Leninskie gory 1/2, Moscow 119991, Russia

Olga Cherkasova

Institute of Laser Physics SB RAS, pr. Lavrentyeva, 13/3, Novosibirsk 630090, Russia

Tatiana Tikhonova

M.V. Lomonosov Moscow State University, International Laser Center, Leninskie gory 1/62, Moscow 119991, Russia

Alexander Priezzhev

M.V. Lomonosov Moscow State University, Physical Department, Leninskie gory 1/2, Moscow 119991, Russia

M.V. Lomonosov Moscow State University, International Laser Center, Leninskie gory 1/62, Moscow 119991, Russia

J. Biomed. Opt. 20(5), 051033 (Feb 18, 2015). doi:10.1117/1.JBO.20.5.051033
History: Received October 15, 2014; Accepted January 16, 2015
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Abstract.  We present the results of a native fluorescence spectroscopy study of blood plasma of rats with experimental diabetes. It was shown that the fluorescence emission band shape at 320 nm excitation is the most indicative of hyperglycemia in the blood plasma samples. We provide the interpretation of this fact based on the changes in reduced nicotinamide adenine dinucleotide phosphate concentration due to glucose-related metabolic pathways and protein fluorescent cross-linking formation following nonenzymatic glycation.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Evgeny Shirshin ; Olga Cherkasova ; Tatiana Tikhonova ; Elena Berlovskaya ; Alexander Priezzhev, et al.
"Native fluorescence spectroscopy of blood plasma of rats with experimental diabetes: identifying fingerprints of glucose-related metabolic pathways", J. Biomed. Opt. 20(5), 051033 (Feb 18, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051033


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