Research Papers: Imaging

Collagen density and alignment in responsive and resistant trastuzumab-treated breast cancer xenografts

[+] Author Affiliations
Alex J. Walsh, Jae H. Lee, Melissa C. Skala

Vanderbilt University, Department of Biomedical Engineering Station B, Box 1631, Nashville, Tennessee 37235, United States

Rebecca S. Cook

Vanderbilt University, Department of Cancer Biology, Breast Cancer Research Program, 2220 Pierce Avenue, Nashville, Tennessee 37232, United States

Carlos L. Arteaga

Vanderbilt University, Department of Cancer Biology, Breast Cancer Research Program, 2220 Pierce Avenue, Nashville, Tennessee 37232, United States

Vanderbilt University, Department of Medicine, 2220 Pierce Avenue, Nashville, Tennessee 37232, United States

J. Biomed. Opt. 20(2), 026004 (Feb 20, 2015). doi:10.1117/1.JBO.20.2.026004
History: Received September 26, 2014; Accepted January 21, 2015
Text Size: A A A

Abstract.  Tumor collagen characteristics influence tumor malignancy, invasion, and metastasis. This study investigates the effects of trastuzumab (Tz) on the collagen of Tz-responsive (BT474) and Tz-resistant (HR6) breast cancer xenografts. Collagen content was assessed by in vivo second harmonic generation (SHG) imaging and histological trichrome staining of tumor sections. Collagen SHG imaging of control BT474 and HR6 tumors demonstrated increased collagen density after 14 days of treatment (p<0.05). Trichrome staining revealed decreased collagen in Tz-treated BT474 and HR6 tumors at 2, 5, and 14 days of treatment, suggesting that Tz affects the tumor microenvironment independent of epithelial cell response. Additionally, collagen alignment analysis revealed significantly less aligned collagen in the Tz-treated BT474 tumors at day 14 compared with control BT474 tumors. There was no correlation between SHG endpoints (collagen density and alignment) and trichrome staining (p>0.05), consistent with the physically distinctive nature of these measurements. There was also no correlation between tumor size and collagen endpoints (p>0.05). These results identify changes within the collagen compartment of the tumor microenvironment following Tz treatment, which are independent from the tumor cell response to Tz, and demonstrate that intravital collagen SHG imaging is capable of measuring dynamic changes in tumor microenvironment following treatment that complements trichrome staining.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Alex J. Walsh ; Rebecca S. Cook ; Jae H. Lee ; Carlos L. Arteaga and Melissa C. Skala
"Collagen density and alignment in responsive and resistant trastuzumab-treated breast cancer xenografts", J. Biomed. Opt. 20(2), 026004 (Feb 20, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.2.026004


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.