Research Papers: Imaging

Enhanced resolution through thick tissue with structured illumination and adaptive optics

[+] Author Affiliations
Benjamin Thomas, Peter Kner

University of Georgia, College of Engineering, 101 Driftmier Engineering Center, Athens, Georgia 30602, United States

Adrian Wolstenholme

University of Georgia, College of Veterinary Medicine, Department of Infectious Diseases, Athens, Georgia 30602, United States

Snehal N. Chaudhari, Edward T. Kipreos

University of Georgia, Department of Cellular Biology, 724 Biological Sciences Building, Athens, Georgia 30602, United States

J. Biomed. Opt. 20(2), 026006 (Feb 25, 2015). doi:10.1117/1.JBO.20.2.026006
History: Received December 10, 2014; Accepted January 30, 2015
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Abstract.  Structured illumination microscopy provides twice the linear resolution of conventional fluorescence microscopy, but in thick samples, aberrations degrade the performance and limit the resolution. Here, we demonstrate structured illumination microscopy through 35 µm of tissue using adaptive optics (AO) to correct aberrations resulting in images with a resolution of 140 nm. We report a 60% minimum improvement in the signal-to-noise ratio of the structured illumination reconstruction through thick tissue by correction with AO.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Benjamin Thomas ; Adrian Wolstenholme ; Snehal N. Chaudhari ; Edward T. Kipreos and Peter Kner
"Enhanced resolution through thick tissue with structured illumination and adaptive optics", J. Biomed. Opt. 20(2), 026006 (Feb 25, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.2.026006


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