Special Section on Laser Applications in Life Sciences

Identification of individual biofilm-forming bacterial cells using Raman tweezers

[+] Author Affiliations
Ota Samek, Silvie Bernatová, Jan Ježek, Martin Šiler, Mojmir Šerý, Vladislav Krzyžánek, Kamila Hrubanová, Pavel Zemánek

Institute of Scientific Instruments of the AS CR, v.v.i., Královopolská 147, 612 64 Brno, Czech Republic

Veronika Holá, Filip Růžička

Masaryk University and St. Anne’s Faculty Hospital, Department of Microbiology, Faculty of Medicine, Pekařská 53, 656 91 Brno, Czech Republic

J. Biomed. Opt. 20(5), 051038 (Mar 02, 2015). doi:10.1117/1.JBO.20.5.051038
History: Received October 9, 2014; Accepted February 5, 2015
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Abstract.  A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral “Raman fingerprints” obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilm-negative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Ota Samek ; Silvie Bernatová ; Jan Ježek ; Martin Šiler ; Mojmir Šerý, et al.
"Identification of individual biofilm-forming bacterial cells using Raman tweezers", J. Biomed. Opt. 20(5), 051038 (Mar 02, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051038


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