Research Papers: Imaging

Photoacoustic lifetime imaging for direct in vivo tissue oxygen monitoring

[+] Author Affiliations
Qi Shao, Shai Ashkenazi

University of Minnesota, Department of Biomedical Engineering, 312 Church Street SE, Minneapolis, Minnesota 55455, United States

J. Biomed. Opt. 20(3), 036004 (Mar 06, 2015). doi:10.1117/1.JBO.20.3.036004
History: Received October 30, 2014; Accepted February 12, 2015
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Abstract.  Measuring the partial pressure of oxygen (pO2) in tissue may provide physicians with essential information about the physiological state of tissue. However, currently available methods for measuring or imaging tissue pO2 have significant limitations, preventing them from being widely used in clinics. Recently, we have reported a direct and noninvasive in vivo imaging modality based on the photoacoustic lifetime which overcomes certain drawbacks of the existing methods. The technique maps the excited triplet state of oxygen-sensitive dye, thus reflecting the spatial and temporal distributions of tissue oxygen. Here, we present two studies which apply photoacoustic lifetime imaging (PALI) to monitor changes of tissue oxygen induced by external modulations. The first study modulates tissue oxygen by controlling the percentage of oxygen a normal mouse inhales. We demonstrate that PALI is able to reflect the change in oxygen level with respect to normal, oxygen-rich, and oxygen-poor breathing conditions. The second study involves an acute ischemia model using a thin thread tied around the hindlimb of a normal mouse to reduce the blood flow. PALI images were acquired before, during, and after the restriction. The drop of tissue pO2 and recovery from hypoxia due to reperfusion were tracked and observed by PALI.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Qi Shao and Shai Ashkenazi
"Photoacoustic lifetime imaging for direct in vivo tissue oxygen monitoring", J. Biomed. Opt. 20(3), 036004 (Mar 06, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.036004


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