Special Section on Laser Applications in Life Sciences

Use of time-resolved fluorescence spectroscopy to evaluate diagnostic value of collagen degradation products

[+] Author Affiliations
Joanna Sikora

Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Cardiology and Internal Medicine, Skłodowskiej-Curie Street No. 9, 85-094 Bydgoszcz, Poland

Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Biochemistry, Karłowicza Street No. 24, 85-094 Bydgoszcz, Poland

Michał Cyrankiewicz, Tomasz Wybranowski, Blanka Ziomkowska, Stefan Kruszewski

Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Department of Biophysics, Jagiellońska Street No. 13, 85-094 Bydgoszcz, Poland

Borys Ośmiałowski

University of Technology and Life Sciences, Faculty of Chemical Technology and Engineering, Department of Organic Chemistry, Seminaryjna Street No. 3, 85-326 Bydgoszcz, Poland

Ewa Obońska, Jacek Kubica

Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Cardiology and Internal Medicine, Skłodowskiej-Curie Street No. 9, 85-094 Bydgoszcz, Poland

Beata Augustyńska

Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Biochemistry, Karłowicza Street No. 24, 85-094 Bydgoszcz, Poland

J. Biomed. Opt. 20(5), 051039 (Mar 12, 2015). doi:10.1117/1.JBO.20.5.051039
History: Received October 21, 2014; Accepted February 23, 2015
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Abstract.  The concentration of collagen degradation products (CDPs) may reflect the process of left ventricular remodeling (LVR). The aim of this study was to evaluate the potential diagnostic usefulness of time-resolved fluorescence spectroscopy (TRFS) in assessment of CDPs. The preliminary experiment was designed to establish if CDPs’ characteristics might be visible by mean fluorescence lifetime (FLT) in determined conditions. The in vitro model of CDPs was prepared by conducting the hydrolysis of type III collagen. The FLT of samples was measured by the time-resolved spectrometer Life Spec II with the subnanosecond pulsed 360-nm EPLED diode. The FLTs were obtained by deconvolution analysis of the data using a multiexponential model of fluorescence decay. In order to determine the limit of traceability of CDPs, a comparison of different collagen/plasma ratio in samples was performed. The results of our study showed that the increase of added plasma to hydrolyzed collagen extended the mean FLT. Thus, the diagnosis of LVR based on measurements using TRFS is possible. However, it is important to point out the experiment was preliminary and further investigation in this field of research is crucial.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Joanna Sikora ; Michał Cyrankiewicz ; Tomasz Wybranowski ; Blanka Ziomkowska ; Borys Ośmiałowski, et al.
"Use of time-resolved fluorescence spectroscopy to evaluate diagnostic value of collagen degradation products", J. Biomed. Opt. 20(5), 051039 (Mar 12, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051039


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