Research Papers: General

Visible micro-Raman spectroscopy of single human mammary epithelial cells exposed to x-ray radiation

[+] Author Affiliations
Ines Delfino

Università della Tuscia, Dipartimento di Scienze Ecologiche e Biologiche, Largo dell’Università snc, Viterbo 01100, Italy

Giuseppe Perna, Maria Lasalvia, Vito Capozzi

Università di Foggia, Dipartimento di Medicina Clinica e Sperimentale, Viale Pinto 1, Foggia 71122, Italy

Lorenzo Manti

Università “Federico II,” Dipartimento di Fisica, Via Cintia, Napoli 80126, Italy

Carlo Camerlingo

Consiglio Nazionale delle Ricerche, CNR-SPIN, UdR di Napoli, Via Campi Flegrei 34, Pozzuoli 80078, Italy

Maria Lepore

Seconda Università di Napoli, Dipartimento di Medicina Sperimentale, Via S.M. Costantinopoli 16, Napoli 80134, Italy

J. Biomed. Opt. 20(3), 035003 (Mar 13, 2015). doi:10.1117/1.JBO.20.3.035003
History: Received November 22, 2014; Accepted February 24, 2015
Text Size: A A A

Abstract.  A micro-Raman spectroscopy investigation has been performed in vitro on single human mammary epithelial cells after irradiation by graded x-ray doses. The analysis by principal component analysis (PCA) and interval-PCA (i-PCA) methods has allowed us to point out the small differences in the Raman spectra induced by irradiation. This experimental approach has enabled us to delineate radiation-induced changes in protein, nucleic acid, lipid, and carbohydrate content. In particular, the dose dependence of PCA and i-PCA components has been analyzed. Our results have confirmed that micro-Raman spectroscopy coupled to properly chosen data analysis methods is a very sensitive technique to detect early molecular changes at the single-cell level following exposure to ionizing radiation. This would help in developing innovative approaches to monitor radiation cancer radiotherapy outcome so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Ines Delfino ; Giuseppe Perna ; Maria Lasalvia ; Vito Capozzi ; Lorenzo Manti, et al.
"Visible micro-Raman spectroscopy of single human mammary epithelial cells exposed to x-ray radiation", J. Biomed. Opt. 20(3), 035003 (Mar 13, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.035003


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.