Research Papers: Imaging

5-aminolevulinic acid induced protoporphyrin IX as a fluorescence marker for quantitative image analysis of high-grade dysplasia in Barrett’s esophagus cellular models

[+] Author Affiliations
Shu-Chi Allison Yeh

McMaster University, School of Biomedical Engineering, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

Samir Sahli

McMaster University, Department of Engineering Physics, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

David W. Andrews

Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, Onario M4N 3M5, Canada

Michael S. Patterson

McMaster University, Department of Medical Physics and Applied Radiation Sciences, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

David Armstrong

McMaster University, Department of Medicine, Division of Gastroenterology, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

John Provias

McMaster University, Department of Pathology and Molecular Medicine, Division of Neuropathology, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

Qiyin Fang

McMaster University, School of Biomedical Engineering, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

McMaster University, Department of Engineering Physics, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada

J. Biomed. Opt. 20(3), 036010 (Mar 19, 2015). doi:10.1117/1.JBO.20.3.036010
History: Received October 18, 2014; Accepted February 23, 2015
Text Size: A A A

Abstract.  Early detection and treatment of high-grade dysplasia (HGD) in Barrett’s esophagus may reduce the risk of developing esophageal adenocarcinoma. Confocal endomicroscopy (CLE) has shown advantages over routine white-light endoscopic surveillance with biopsy for histological examination; however, CLE is compromised by insufficient contrast and by intra- and interobserver variation. An FDA-approved PDT photosensitizer was used here to reveal morphological and textural features similar to those found in histological analysis. Support vector machines were trained using the aforementioned features to obtain an automatic and robust detection of HGD. Our results showed 95% sensitivity and 87% specificity using the optimal feature combination and demonstrated the potential for extension to a three-dimensional cell model.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Shu-Chi Allison Yeh ; Samir Sahli ; David W. Andrews ; Michael S. Patterson ; David Armstrong, et al.
"5-aminolevulinic acid induced protoporphyrin IX as a fluorescence marker for quantitative image analysis of high-grade dysplasia in Barrett’s esophagus cellular models", J. Biomed. Opt. 20(3), 036010 (Mar 19, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.036010


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.