Research Papers: Imaging

Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles

[+] Author Affiliations
Yuan Liu, Ming-Yuan Wei, Baohong Yuan

The University of Texas at Arlington, Department of Bioengineering, 500 UTA Boulevard, Arlington, Texas 76010, United States

The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, Joint Biomedical Engineering Program, 5323 Harry Hines Boulevard, Dallas, Texas 75390, United States

Jameel A. Feshitan, Mark A. Borden

University of Colorado, Department of Mechanical Engineering, 1111 Engineering Drive, Boulder, Colorado 80309-0427, United States

J. Biomed. Opt. 20(3), 036012 (Mar 19, 2015). doi:10.1117/1.JBO.20.3.036012
History: Received October 9, 2014; Accepted March 3, 2015
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Abstract.  A fluorescence resonance energy transfer (FRET)-based microbubble contrast agent system was designed to experimentally demonstrate the concept of ultrasound-modulated fluorescence (UMF). Microbubbles were simultaneously labeled with donor and acceptor fluorophores on the surface to minimize self-quenching and maximize FRET. In response to ultrasound, the quenching efficiency was greatly modulated by changing the distance between the donor and acceptor molecules through microbubble size oscillations. Both donors and acceptors exhibited UMF on individual microbubbles. The UMF strength of the donor was more significant compared to that of the acceptor. Furthermore, the UMF of the donor was observed from a microbubble solution in a turbid media. This study exploits the feasibility of donor–acceptor labeled microbubbles as UMF contrast agents.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Yuan Liu ; Jameel A. Feshitan ; Ming-Yuan Wei ; Mark A. Borden and Baohong Yuan
"Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles", J. Biomed. Opt. 20(3), 036012 (Mar 19, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.036012


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