Research Papers: Therapeutic

Alveolar bone dynamics in osteoporotic rats treated with raloxifene or alendronate: confocal microscopy analysis

[+] Author Affiliations
Gabriel Ramalho-Ferreira, Leonardo Perez Faverani, Gustavo Augusto Grossi-Oliveira, Tetuo Okamoto

Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Surgery and Integrated Clinic, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil

Roberta Okamoto

Universidade Estadual Paulista (UNESP), Araçatuba Dental School, Department of Basic Sciences, José Bonifácio, 1193, Aracatuba, Sao Paulo 16015-050, Brazil

J. Biomed. Opt. 20(3), 038003 (Mar 26, 2015). doi:10.1117/1.JBO.20.3.038003
History: Received November 10, 2014; Accepted March 6, 2015
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Abstract.  In this study, the characteristics of the alveolar bone of rats with induced osteoporosis were examined. Thirty-two rats were divided into four groups according to the induction of osteoporosis and drugs administered: OG, osteoporotic rats without treatment (negative control); SG, rats which underwent sham surgery ovariectomy (SHAM); alendronate (AG), osteoporotic rats treated with alendronate; and RG, osteoporotic rats treated with raloxifene (RG). On the 8th day after ovariectomy and SHAM surgeries, drug therapy was started with AG or RG. On the 52nd day, 20mg/kg calcein was administered to all of the rats, and on the 80th day, 20mg/kg alizarin red was administered. Euthanasia was performed on the 98th day. The bone area marked by fluorochromes was calculated and data were subjected to two-way ANOVA test and Tukey’s post-hoc test (p<0.05). The comparison of the induced osteoporosis groups showed no statistically significant differences in bone turnover only between RG and SG (p=0.074) and AG and OG (p=0.138). All other comparisons showed significant differences (p<0.001). The largest bone turnover was observed in RG and SG groups. RG was the medication that improved the dynamics of the alveolar bone of rats with induced osteoporosis, resembling that of healthy rats.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Gabriel Ramalho-Ferreira ; Leonardo Perez Faverani ; Gustavo Augusto Grossi-Oliveira ; Tetuo Okamoto and Roberta Okamoto
"Alveolar bone dynamics in osteoporotic rats treated with raloxifene or alendronate: confocal microscopy analysis", J. Biomed. Opt. 20(3), 038003 (Mar 26, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.038003


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