Research Papers: Imaging

Spectrally encoded slit confocal microscopy using a wavelength-swept laser

[+] Author Affiliations
Soocheol Kim, Jaehyun Hwang, Jung Heo, Suho Ryu, Donghak Lee, Chulmin Joo

Yonsei University, School of Mechanical Engineering, Seoul 120-749, Republic of Korea

Sang-Hoon Kim, Seung Jae Oh

Yonsei University, YUHS-KRIBB Medical Convergence Research Institute, Severance Biomedical Science Institute, and Department of Radiology, Seoul 120-749, Republic of Korea

J. Biomed. Opt. 20(3), 036016 (Mar 26, 2015). doi:10.1117/1.JBO.20.3.036016
History: Received October 23, 2014; Accepted March 10, 2015
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Abstract.  We present an implementation of spectrally encoded slit confocal microscopy. The method employs a rapid wavelength-swept laser as the light source and illuminates a specimen with a line focus that scans through the specimen as the wavelength sweeps. The reflected light from the specimen is imaged with a stationary line scan camera, in which the finite pixel height serves as a slit aperture. This scanner-free operation enables a simple and cost-effective implementation in a small form factor, while allowing for the three-dimensional imaging of biological samples.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Soocheol Kim ; Jaehyun Hwang ; Jung Heo ; Suho Ryu ; Donghak Lee, et al.
"Spectrally encoded slit confocal microscopy using a wavelength-swept laser", J. Biomed. Opt. 20(3), 036016 (Mar 26, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.036016


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