Research Papers: Imaging

Imaging of nanoparticle-labeled stem cells using magnetomotive optical coherence tomography, laser speckle reflectometry, and light microscopy

[+] Author Affiliations
Peter Cimalla, Theresa Werner, Kai Winkler, Maria Gaertner, Edmund Koch

Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany

Claudia Mueller, Dierk Wittig

Technische Universität Dresden, Institute of Anatomy, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, Germany

Life Science Inkubator GmbH, Ludwig-Erhard-Allee 2, 53175 Bonn, Germany

Sebastian Wicht, Bernd Rellinghaus

IFW Dresden, Institute for Metallic Materials, Helmholtzstraße 20, 01069 Dresden, Germany

Mirko Mehner, Julia Walther

Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstrasse 74, 01307 Dresden, Germany

Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Medizinische Physik und Biomedizinische Technik, Fetscherstrasse 74, 01307 Dresden, Germany

Mike O. Karl

Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden (CRTD), Fetscherstraße 105, 01307 Dresden, Germany

German Center for Neurodegenerative Diseases (DZNE), Arnoldstraße 18, 01307 Dresden, Germany

Marius Ader

Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden (CRTD), Fetscherstraße 105, 01307 Dresden, Germany

Richard H. W. Funk

Technische Universität Dresden, Institute of Anatomy, Faculty of Medicine Carl Gustav Carus, Fetscherstrasse 74, 01307 Dresden, Germany

J. Biomed. Opt. 20(3), 036018 (Mar 30, 2015). doi:10.1117/1.JBO.20.3.036018
History: Received December 12, 2014; Accepted March 12, 2015
Text Size: A A A

Abstract.  Cell transplantation and stem cell therapy are promising approaches for regenerative medicine and are of interest to researchers and clinicians worldwide. However, currently, no imaging technique that allows three-dimensional in vivo inspection of therapeutically administered cells in host tissues is available. Therefore, we investigate magnetomotive optical coherence tomography (MM-OCT) of cells labeled with magnetic particles as a potential noninvasive cell tracking method. We develop magnetomotive imaging of mesenchymal stem cells for future cell therapy monitoring. Cells were labeled with fluorescent iron oxide nanoparticles, embedded in tissue-mimicking agar scaffolds, and imaged using a microscope setup with an integrated MM-OCT probe. Magnetic particle-induced motion in response to a pulsed magnetic field of 0.2 T was successfully detected by OCT speckle variance analysis, and cross-sectional and volumetric OCT scans with highlighted labeled cells were obtained. In parallel, fluorescence microscopy and laser speckle reflectometry were applied as two-dimensional reference modalities to image particle distribution and magnetically induced motion inside the sample, respectively. All three optical imaging modalities were in good agreement with each other. Thus, magnetomotive imaging using iron oxide nanoparticles as cellular contrast agents is a potential technique for enhanced visualization of selected cells in OCT.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Peter Cimalla ; Theresa Werner ; Kai Winkler ; Claudia Mueller ; Sebastian Wicht, et al.
"Imaging of nanoparticle-labeled stem cells using magnetomotive optical coherence tomography, laser speckle reflectometry, and light microscopy", J. Biomed. Opt. 20(3), 036018 (Mar 30, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.3.036018


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.