Research Papers: Imaging

Usage of polarization-sensitive optical coherence tomography for investigation of collagen cross-linking

[+] Author Affiliations
Myeong Jin Ju, Shuo Tang

University of British Columbia, Department of Electrical and Computer Engineering, 2332 Main Mall, Vancouver, British Columbia V6T 1Z4, Canada

J. Biomed. Opt. 20(4), 046001 (Apr 02, 2015). doi:10.1117/1.JBO.20.4.046001
History: Received December 25, 2014; Accepted March 16, 2015
Text Size: A A A

Abstract.  To investigate morphological alternation in corneal stroma induced by collagen cross-linking (CXL) treatment, polarization-sensitive optical coherence tomography (PS-OCT) capable of providing scattering, phase retardation, and degree of polarization uniformity (DOPU) images were employed on fresh bovine cornea. Significant corneal thickness reduction was observed after the CXL procedure, and its variation was quantitatively analyzed. From the scattering contrast, a hyperscattering region was observed in the anterior of the cornea immediately after the CXL procedure and its range increased with time. Within the scattering region, a slow increase was observed in the phase retardation image, and a discriminable characteristic was found in the DOPU image. A global threshold value was empirically determined from the averaged DOPU depth profile in order to locate the effective cross-linking depth. In addition to the standard protocol, an accelerated CXL procedure shortening the treatment time with higher intensity of ultraviolet-A (UV-)A power was also performed. From the measurement results after the two different CXL protocols, different cross-linking aspects were found and their difference was discussed in terms of the effectiveness of cross-linking. Based on this study, we believe that PS-OCT could be a promising optical imaging modality to evaluate the progression and effectiveness of the riboflavin/UV-A induced corneal collagen cross-linking.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Myeong Jin Ju and Shuo Tang
"Usage of polarization-sensitive optical coherence tomography for investigation of collagen cross-linking", J. Biomed. Opt. 20(4), 046001 (Apr 02, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.4.046001


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.