With these limitations in mind, it is clear from the data that, depending on the sample, scanning two-photon microscopy can achieve approximately twice the penetration depth of temporal focusing when subject to practical issues such as sample damage (see Figs. 4 and 5, and the data summary in Fig. 6). It was also possible to compare organs in terms of the achievable penetration depth; for the previously mentioned maximum spatial frequency of , the heart consistently demonstrated the lowest penetration depth, around for temporal focusing and for scanning two-photon microscopy. The lungs, liver, and kidneys were all very similar, with penetration depths of approximately for temporal focusing and for scanning two-photon microscopy. The spleen is slightly easier to penetrate, at around for temporal focusing and for scanning two-photon microscopy, but the low absorption and scattering due to the large lipid droplets in white adipose tissue meant that penetration depths of nearly for temporal focusing and over for scanning two-photon microscopy were possible. The especially large error bars were due to the large variation in the measured scanning two-photon penetration depths; values of 190, 635, and were recorded, and the result strongly skewed the results. This extremely large variation was, in turn, caused primarily by the sparse staining of the sample; the higher resolution of the temporal focusing image made it possible to more readily locate the nuclei, whereas the lower resolution and smaller FOV of the raster-scanning two-photon image meant that the compressed nuclei were harder to distinguish.