Research Papers: Imaging

In vitro colocalization of plasmonic nano-biolabels and biomolecules using plasmonic and Raman scattering microspectroscopy

[+] Author Affiliations
Kamalesh Chaudhari, Thalappil Pradeep

Indian Institute of Technology Madras, Department of Chemistry, DST Unit of Nanoscience and Thematic Unit of Excellence, Chennai 600 036, India

J. Biomed. Opt. 20(4), 046011 (Apr 22, 2015). doi:10.1117/1.JBO.20.4.046011
History: Received January 5, 2015; Accepted March 20, 2015
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Abstract.  An insight into the intracellular fate of theranostics is important for improving their potential in biological applications. In vivo efficacy of plasmonic theranostics depends on our ability to monitor temporal changes in their size, shape, and state of aggregation, and the identification of molecules adsorbed on their surfaces. We develop a technique which combines plasmonic and Raman scattering microspectroscopy to colocalize plasmonic scattering from metallic nanoparticles with the Raman signatures of biomolecules adsorbed on the surface of the former. Using this technique, we have colocalized biomolecules with the plasmonic scattering from silver nanoparticles in the vicinity of Escherichia coli bacteria. To prove the applicability of this setup for the measurements on mammalian cells, imaging of HEK293 cells treated with gold nanoparticles was performed. We discuss the importance of such correlated measurements over individual techniques, although the latter may lead to misinterpretation of results. Finally, with the above-mentioned examples, we have given criteria to improve the specificity of theranostics. We believe that this methodology will be considered as a prime development in the assessment of theranostics.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Kamalesh Chaudhari and Thalappil Pradeep
"In vitro colocalization of plasmonic nano-biolabels and biomolecules using plasmonic and Raman scattering microspectroscopy", J. Biomed. Opt. 20(4), 046011 (Apr 22, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.4.046011


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