Research Papers: Sensing

Raman microspectroscopy of nanodiamond-induced structural changes in albumin

[+] Author Affiliations
Anastasiya S. Svetlakova, Nikolay N. Brandt

Lomonosov Moscow State University, Physics Department, Leninskie Gory 1/62, Moscow 119991, Russia

Alexander V. Priezzhev

Lomonosov Moscow State University, Physics Department, Leninskie Gory 1/62, Moscow 119991, Russia

Lomonosov Moscow State University, International Laser Center, Leninskie Gory 1/62, Moscow 119992, Russia

Andrey Yu. Chikishev

Lomonosov Moscow State University, International Laser Center, Leninskie Gory 1/62, Moscow 119992, Russia

J. Biomed. Opt. 20(4), 047004 (Apr 22, 2015). doi:10.1117/1.JBO.20.4.047004
History: Received October 22, 2014; Accepted March 30, 2015
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Abstract.  Nanodiamonds (NDs) are promising agents for theranostic applications due to reported low toxicity and high biocompatibility, which is still being extensively tested on cellular, tissue, and organism levels. It is presumed that for experimental and future clinical applications, NDs will be administered into the organism via the blood circulation system. In this regard, the interaction of NDs with blood components needs to be thoroughly studied. We studied the interaction of carboxylated NDs (cNDs) with albumin, one of the major proteins of blood plasma. After 2-h long in vitro incubation in an aqueous solution of the protein, 100-nm cNDs were dried and the dry samples were studied with the aid of Raman microspectroscopy. The spectroscopic data indicate significant conformational changes that can be due to cND–protein interaction. A possible decrease in the functional activity of albumin related to the conformational changes must be taken into account in the in vivo applications.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Anastasiya S. Svetlakova ; Nikolay N. Brandt ; Alexander V. Priezzhev and Andrey Yu. Chikishev
"Raman microspectroscopy of nanodiamond-induced structural changes in albumin", J. Biomed. Opt. 20(4), 047004 (Apr 22, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.4.047004


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