Research Papers: Imaging

Rare-earth-doped nanophosphors for multicolor cathodoluminescence nanobioimaging using scanning transmission electron microscopy

[+] Author Affiliations
Taichi Furukawa

Osaka University, Institute for NanoScience Design, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan

Shoichiro Fukushima, Hirohiko Niioka, Jun Miyake, Tsutomu Araki, Mamoru Hashimoto

Osaka University, Graduate School of Engineering Science, Department of Mechanical Science and Bioengineering, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan

Naoki Yamamoto

Tokyo Institute of Technology, Department of Physics, Oh-okayama, Meguro-ku, Tokyo 152-8551, Japan

J. Biomed. Opt. 20(5), 056007 (May 22, 2015). doi:10.1117/1.JBO.20.5.056007
History: Received February 4, 2015; Accepted April 17, 2015
Text Size: A A A

Abstract.  We describe rare-earth-doped nanophosphors (RE-NPs) for biological imaging using cathodoluminescence (CL) microscopy based on scanning transmission electron microscopy (STEM). We report the first demonstration of multicolor CL nanobioimaging using STEM with nanophosphors. The CL spectra of the synthesized nanophosphors (Y2O3:Eu, Y2O3:Tb) were sufficiently narrow to be distinguished. From CL images of RE-NPs on an elastic carbon-coated copper grid, the spatial resolution was beyond the diffraction limit of light. Y2O3:Tb and Y2O3:Eu RE-NPs showed a remarkable resistance against electron beam exposure even at high acceleration voltage (80 kV) and retained a CL intensity of more than 97% compared with the initial intensity for 1 min. In biological CL imaging with STEM, heavy-metal-stained cell sections containing the RE-NPs were prepared, and both the CL images of RE-NPs and cellular structures, such as mitochondria, were clearly observed from STEM images with high contrast. The cellular CL imaging using RE-NPs also had high spatial resolution even though heavy-metal-stained cells are normally regarded as highly scattering media. Moreover, since the RE-NPs exhibit photoluminescence (PL) excited by UV light, they are useful for multimodal correlative imaging using CL and PL.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Taichi Furukawa ; Shoichiro Fukushima ; Hirohiko Niioka ; Naoki Yamamoto ; Jun Miyake, et al.
"Rare-earth-doped nanophosphors for multicolor cathodoluminescence nanobioimaging using scanning transmission electron microscopy", J. Biomed. Opt. 20(5), 056007 (May 22, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.056007


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.