JBO Letters

Photoacoustic imaging of human lymph nodes with endogenous lipid and hemoglobin contrast

[+] Author Affiliations
James A. Guggenheim, Thomas J. Allen, Edward Z. Zhang, Paul C. Beard

University College London, Department of Medical Physics and Biomedical Engineering, Gower Street, London WC1E 6BT, United Kingdom

Andrew Plumb, Shonit Punwani

University College London, Centre for Medical Imaging, 3rd Floor East, 250 Euston Road, London NW1 2PG, United Kingdom

Manuel Rodriguez-Justo

University College London, Cancer Institute, Department of Research Pathology, Rockefeller Building, 21 University Street, London WC1E 6JJ, United Kingdom

J. Biomed. Opt. 20(5), 050504 (May 26, 2015). doi:10.1117/1.JBO.20.5.050504
History: Received February 26, 2015; Accepted April 22, 2015
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Abstract.  Lymph nodes play a central role in metastatic cancer spread and are a key clinical assessment target. Abnormal node vascularization, morphology, and size may be indicative of disease but can be difficult to visualize with sufficient accuracy using existing clinical imaging modalities. To explore the potential utility of photoacoustic imaging for the assessment of lymph nodes, images of ex vivo samples were obtained at multiple wavelengths using a high-resolution three-dimensional photoacoustic scanner. These images showed that hemoglobin based contrast reveals nodal vasculature and lipid-based contrast reveals the exterior node size, shape, and boundary integrity. These two sources of complementary contrast may allow indirect observation of cancer, suggesting a future role for photoacoustic imaging as a tool for the clinical assessment of lymph nodes.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

James A. Guggenheim ; Thomas J. Allen ; Andrew Plumb ; Edward Z. Zhang ; Manuel Rodriguez-Justo, et al.
"Photoacoustic imaging of human lymph nodes with endogenous lipid and hemoglobin contrast", J. Biomed. Opt. 20(5), 050504 (May 26, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.050504


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