Special Section on Laser Applications in Life Sciences

Role of 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer

[+] Author Affiliations
Zhenxi Zhang, Sijia Wang, Hao Xu, Bo Wang, Cuiping Yao

Xi’an Jiaotong University, Institute of Biomedical Analytical Technology and Instrumentation, School of Life Science and Technology, Key Laboratory of Biomedical Information Engineering of Ministry of Education, 28 Xianning Xi Road, Xi’an, Shaanxi 710049, China

J. Biomed. Opt. 20(5), 051043 (May 28, 2015). doi:10.1117/1.JBO.20.5.051043
History: Received October 20, 2014; Accepted May 5, 2015
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Abstract.  There are three possible mechanisms for 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (GNPs) through electrostatic bonding for photodynamic therapy (PDT) of cancer: GNPs delivery function, singlet oxygen generation (SOG) by GNPs irradiated by light, and surface resonance enhancement (SRE) of SOG. Figuring out the exact mechanism is important for further clinical treatment. 5-ALA-GNPs and human chronic myeloid leukemia K562 cells were used to study delivery function and SOG by GNPs. The SRE of SOG enabled by GNPs was explored by protoporphyrin IX (PpIX)-GNPs conjugate through electrostatic bonding. Cell experiments show that the GNPs can improve the efficiency of PDT, which is due to the vehicle effect of GNPs. PpIX–GNPs conjugate experiments demonstrated that SOG can be improved about 2.5 times over PpIX alone. The experiments and theoretical results show that the local field enhancement (LFE) via localized surface plasmon resonance (LSPR) of GNPs is the major role; the LFE was dependent on the irradiation wavelength and the GNP’s size. The LFE increased with an increase of the GNP size (2R 50nm). However, the LSPR function of the GNPs was not found in cell experiments. Our study shows that in 5-ALA-conjugated GNPs PDT, the delivery function of GNPs is the major role.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Zhenxi Zhang ; Sijia Wang ; Hao Xu ; Bo Wang and Cuiping Yao
"Role of 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer", J. Biomed. Opt. 20(5), 051043 (May 28, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.051043


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