Research Papers: Imaging

Ex vivo characterization of normal and adenocarcinoma colon samples by Mueller matrix polarimetry

[+] Author Affiliations
Iftikhar Ahmad

Pakistan Institute of Engineering and Applied Sciences, Department of Physics and Applied Mathematics, Nilore, Islamabad 45650, Pakistan

Center for Nuclear Medicine and Radiotherapy (CENAR), Brewery Road, 17, Quetta, Pakistan

Manzoor Ahmad

Pakistan Institute of Engineering and Applied Sciences, Department of Physics and Applied Mathematics, Nilore, Islamabad 45650, Pakistan

Islamia College (University), Department of Physics, Peshawar, Pakistan

Karim Khan, Sumara Ashraf, Masroor Ikram

Pakistan Institute of Engineering and Applied Sciences, Department of Physics and Applied Mathematics, Nilore, Islamabad 45650, Pakistan

Shakil Ahmad

Swat Institute of Nuclear Medicine, Oncology and Radiotherapy (SINOR), Saidu Sharif, 50, Swat, Pakistan

J. Biomed. Opt. 20(5), 056012 (May 28, 2015). doi:10.1117/1.JBO.20.5.056012
History: Received December 8, 2014; Accepted May 4, 2015
Text Size: A A A

Abstract.  Mueller matrix polarimetry along with polar decomposition algorithm was employed for the characterization of ex vivo normal and adenocarcinoma human colon tissues by polarized light in the visible spectral range (425–725 nm). Six derived polarization metrics [total diattenuation (DT), retardance (RT), depolarization (ΔT), linear diattenuation (DL), retardance (δ), and depolarization (ΔL)] were compared for normal and adenocarcinoma colon tissue samples. The results show that all six polarimetric properties for adenocarcinoma samples were significantly higher as compared to the normal samples for all wavelengths. The Wilcoxon rank sum test illustrated that total retardance is a good candidate for the discrimination of normal and adenocarcinoma colon samples. Support vector machine classification for normal and adenocarcinoma based on the four polarization properties spectra (ΔT, ΔL, RT,and δ) yielded 100% accuracy, sensitivity, and specificity, while both DT and DL showed 66.6%, 33.3%, and 83.3% accuracy, sensitivity, and specificity, respectively. The combination of polarization analysis and given classification methods provides a framework to distinguish the normal and cancerous tissues.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Iftikhar Ahmad ; Manzoor Ahmad ; Karim Khan ; Sumara Ashraf ; Shakil Ahmad, et al.
"Ex vivo characterization of normal and adenocarcinoma colon samples by Mueller matrix polarimetry", J. Biomed. Opt. 20(5), 056012 (May 28, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.5.056012


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.