Research Papers: Imaging

Label-free and depth resolved optical sectioning of iron-complex deposits in sickle cell disease splenic tissue by multiphoton microscopy

[+] Author Affiliations
Genevieve D. Vigil, Tahsin Ahmed, Aamir Khan, Scott S. Howard

University of Notre Dame, Department of Electrical Engineering, 275 Fitzpatrick Hall, Notre Dame, Indiana 46556, United States

Alexander J. Adami, Roger S. Thrall

University of Connecticut Health Center, Department of Immunology, 263 Farmington Avenue, Farmington, Connecticut 06030-3710, United States

Sarah Chapman

University of Notre Dame, Notre Dame Integrated Imaging Facility Histology Core, 400 D Friemann Life Science Center, Notre Dame, Indiana 46556, United States

Biree Andemariam

University of Connecticut Health Center, Department of Medicine, 263 Farmington Avenue, Farmington, Connecticut 06030-3710, United States

J. Biomed. Opt. 20(6), 066001 (Jun 04, 2015). doi:10.1117/1.JBO.20.6.066001
History: Received October 2, 2014; Accepted April 21, 2015
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Abstract.  Multiphoton microscopy (MPM) imaging of intrinsic two-photon excited fluorescence (TPEF) is performed on humanized sickle cell disease (SCD) mouse model splenic tissue. Distinct morphological and spectral features associated with SCD are identified and discussed in terms of diagnostic relevance. Specifically, spectrally unique splenic iron-complex deposits are identified by MPM; this finding is supported by TPEF spectroscopy and object size to standard histopathological methods. Further, iron deposits are found at higher concentrations in diseased tissue than in healthy tissue by all imaging methods employed here including MPM, and therefore, may provide a useful biomarker related to the disease state. These newly characterized biomarkers allow for further investigations of SCD in live animals as a means to gain insight into the mechanisms impacting immune dysregulation and organ malfunction, which are currently not well understood.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Genevieve D. Vigil ; Alexander J. Adami ; Tahsin Ahmed ; Aamir Khan ; Sarah Chapman, et al.
"Label-free and depth resolved optical sectioning of iron-complex deposits in sickle cell disease splenic tissue by multiphoton microscopy", J. Biomed. Opt. 20(6), 066001 (Jun 04, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.066001


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