Research Papers: Imaging

Development and validation of a custom made indocyanine green fluorescence lymphatic vessel imager

[+] Author Affiliations
Olivia J. Pallotta, Mark McEwen, Lynne Burrow, Jack Beesley

South Australian Biomedical Engineering at Flinders Medical Centre, Flinders Biomedical Engineering Department, Research & Teaching, Bedford Park SA 5042, Australia

Malou van Zanten, Neil Piller

Flinders University, Department of Surgery, Lymphoedema Research Unit, Level 3, Flinders Medical Centre, Bedford Park, SA 5042, Australia

J. Biomed. Opt. 20(6), 066003 (Jun 09, 2015). doi:10.1117/1.JBO.20.6.066003
History: Received March 2, 2015; Accepted May 12, 2015
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Abstract.  Lymphoedema is a chronic progressive condition often producing significant morbidity. An in-depth understanding of an individual’s lymphatic architecture is valuable both in the understanding of underlying pathology and for targeting and tailoring treatment. Severe lower limb injuries resulting in extensive loss of soft tissue require transposition of a flap consisting of muscle and/or soft tissue to close the defect. These patients are at risk of lymphoedema and little is known about lymphatic regeneration within the flap. Indocyanine green (ICG), a water-soluble dye, has proven useful for the imaging of lymphatic vessels. When injected into superficial tissues it binds to plasma proteins in lymph. By exposing the dye to specific wavelengths of light, ICG fluoresces with near-infrared light. Skin is relatively transparent to ICG fluorescence, enabling the visualization and characterization of superficial lymphatic vessels. An ICG fluorescence lymphatic vessel imager was manufactured to excite ICG and visualize real-time fluorescence as it travels through the lymphatic vessels. Animal studies showed successful ICG excitation and detection using this imager. Clinically, the imager has assisted researchers to visualize otherwise hidden superficial lymphatic pathways in patients postflap surgery. Preliminary results suggest superficial lymphatic vessels do not redevelop in muscle flaps.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Olivia J. Pallotta ; Malou van Zanten ; Mark McEwen ; Lynne Burrow ; Jack Beesley, et al.
"Development and validation of a custom made indocyanine green fluorescence lymphatic vessel imager", J. Biomed. Opt. 20(6), 066003 (Jun 09, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.066003


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