Research Papers: Sensing

Incubator proof miniaturized Holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections

[+] Author Affiliations
Beatrix Peter, Judit Nador, Krisztina Juhasz, Daniel Patko, Robert Horvath

University of Pannonia, Doctoral School of Molecular and Nanotechnologies, 8200 Veszprém, Hungary

Nanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary

Agnes Dobos, Inna Székács

Nanobiosensorics Group, Hungarian Academy of Sciences, Institute for Technical Physics and Materials Science, 1121 Budapest, Hungary

Laszlo Korosi

Enviroinvest Ltd., 7632 Pécs, Hungary

J. Biomed. Opt. 20(6), 067002 (Jun 09, 2015). doi:10.1117/1.JBO.20.6.067002
History: Received April 3, 2015; Accepted May 7, 2015
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Abstract.  The in situ observation of cell movements and morphological parameters over longer periods of time under physiological conditions is critical in basic cell research and biomedical applications. The quantitative phase-contrast microscope applied in this study has a remarkably small size, therefore it can be placed directly into a humidified incubator. Here, we report on the successful application of this M4 Holomonitor to observe cancer cell motility, motility speed, and migration in the presence of the green tea polyphenol, epigallocatechin gallate, as well as to monitor the adhesion of preosteoblast cells on nanostructured titanate coatings, relevant for biomedical applications. A special mechanical stage was developed to position the sample into that range of the optical arrangement where digital autofocusing works with high reproducibility and precision. By in-depth analyzing the obtained single cell morphological parameters, we show that the limited vertical resolution of the optical setup results in underestimated single cell contact area and volume and overestimated single cell averaged thickness. We propose a simple model to correct the recorded data to obtain more precise single cell parameters. We compare the results with the kinetic data recorded by a surface sensitive optical biosensor, optical waveguide lightmode spectroscopy.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Beatrix Peter ; Judit Nador ; Krisztina Juhasz ; Agnes Dobos ; Laszlo Korosi, et al.
"Incubator proof miniaturized Holomonitor to in situ monitor cancer cells exposed to green tea polyphenol and preosteoblast cells adhering on nanostructured titanate surfaces: validity of the measured parameters and their corrections", J. Biomed. Opt. 20(6), 067002 (Jun 09, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.067002


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