Research Papers: Imaging

En face Doppler total retinal blood flow measurement with 70 kHz spectral optical coherence tomography

[+] Author Affiliations
Ou Tan, Gangjun Liu, Liu Liang, Simon S. Gao, Alex D. Pechauer, Yali Jia, David Huang

Oregon Health and Science University, Casey Eye Institute, 3375 NW Terwilliger Boulevard, Portland, Oregon 97239, United States

J. Biomed. Opt. 20(6), 066004 (Jun 10, 2015). doi:10.1117/1.JBO.20.6.066004
History: Received February 20, 2015; Accepted May 14, 2015
Text Size: A A A

Abstract.  An automated algorithm was developed for total retinal blood flow (TRBF) using 70-kHz spectral optical coherence tomography (OCT). The OCT was calibrated for the transformation from Doppler shift to speed based on a flow phantom. The TRBF scan pattern contained five repeated volume scans (2×2mm) obtained in 3 s and centered on central retinal vessels in the optic disc. The TRBF was calculated using an en face Doppler technique. For each retinal vein, blood flow was measured at an optimal plane where the calculated flow was maximized. The TRBF was calculated by summing flow in all veins. The algorithm tracked vascular branching so that either root or branch veins are summed, but never both. The TRBF in five repeated volumes were averaged to reduce variation due to cardiac cycle pulsation. Finally, the TRBF was corrected for eye length variation. Twelve healthy eyes and 12 glaucomatous eyes were enrolled to test the algorithm. The TRBF was 45.4±6.7μl/min for healthy control and 34.7±7.6μl/min for glaucomatous participants (p-value=0.01). The intravisit repeatability was 8.6% for healthy controls and 8.4% for glaucoma participants. The proposed automated method provided repeatable TRBF measurement.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Ou Tan ; Gangjun Liu ; Liu Liang ; Simon S. Gao ; Alex D. Pechauer, et al.
"En face Doppler total retinal blood flow measurement with 70 kHz spectral optical coherence tomography", J. Biomed. Opt. 20(6), 066004 (Jun 10, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.066004


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.