Research Papers: Sensing

Fluorescence lifetime of normal, benign, and malignant thyroid tissues

[+] Author Affiliations
Mariana Brandao, Kaique Haleplian, Amando Ito, Luciano Bachmann

Universidade de São Paulo, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Departamento de Física. Avenue Bandeirantes 3900, Ribeirão Preto, São Paulo 14040-901, Brazil

Ricardo Iwakura, Fagne Basilio, Luiz Carlos Conti de Freitas

Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Oftalmo, Otorrino e Cirurgia de Cabeça e Pescoço, Avenue Bandeirantes 3900, Ribeirão Preto, São Paulo 14040-901, Brazil

J. Biomed. Opt. 20(6), 067003 (Jun 17, 2015). doi:10.1117/1.JBO.20.6.067003
History: Received February 12, 2015; Accepted May 19, 2015
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Abstract.  Fine-needle aspiration cytology is the standard technique to diagnose thyroid pathologies. However, this method results in a high percentage of inconclusive and false negatives. The use of time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and nondestructive method to assist during surgical procedures. This study aimed to use fluorescence lifetimes to differentiate healthy and benign tissues from malignant thyroid tissue. The thyroid tissue was excited at 298–300 nm and the fluorescence decay registered at 340 and 450 nm. We observed fluorescence lifetimes at 340 nm emission of 0.80±0.26 and 3.94±0.47ns for healthy tissue; 0.90±0.24 and 4.05±0.46ns for benign lesions; and 1.21±0.14 and 4.63±0.25ns for malignant lesions. For 450 nm emissions, we obtain lifetimes of 0.25±0.18 and 3.99±0.39ns for healthy tissue, 0.24±0.17 and 4.20±0.48ns for benign lesions, 0.33±0.32 and 4.55±0.55ns for malignant lesions. Employing analysis of variance, we differentiate malignant lesions from benign and healthy tissues. In addition, we use quadratic discriminant analysis to distinguish malignant from benign and healthy tissues with an accuracy of 76.1%, sensitivity of 74.7%, and specificity of 83.3%. These results indicate that time-resolved fluorescence can assist medical evaluation of thyroid pathologies during surgeries.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Mariana Brandao ; Ricardo Iwakura ; Fagne Basilio ; Kaique Haleplian ; Amando Ito, et al.
"Fluorescence lifetime of normal, benign, and malignant thyroid tissues", J. Biomed. Opt. 20(6), 067003 (Jun 17, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.067003


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