Research Papers: Imaging

Determination of biodistribution of ultrasmall, near-infrared emitting gold nanoparticles by photoacoustic and fluorescence imaging

[+] Author Affiliations
Wilson Poon

McGill University, Department of Biomedical Engineering, 3775 University Street, Montréal, Quebec H3A 2B4, Canada

University of Toronto, Institute of Biomaterials and Biomedical Engineering, 160 College Street, Toronto, Ontario M5S 3E1, Canada

Andrew Heinmiller

VisualSonics Inc., 6100-3080 Yonge Street, Toronto, Ontario M4N 3N1, Canada

Xuan Zhang, Jay L. Nadeau

McGill University, Department of Biomedical Engineering, 3775 University Street, Montréal, Quebec H3A 2B4, Canada

J. Biomed. Opt. 20(6), 066007 (Jun 23, 2015). doi:10.1117/1.JBO.20.6.066007
History: Received January 26, 2015; Accepted May 28, 2015
Text Size: A A A

Abstract.  This study compares fluorescence and photoacoustic (PA) imaging of ex vivo tumors and organs from tumor-bearing mice injected intravenously with ultrasmall (<3nm) tiopronin-capped Au nanoparticles and compares the data with inductively coupled plasma mass spectrometry (ICP-MS). Good agreement is seen in particle distributions and concentrations at the organ level. The spatial resolution from the imaging techniques allows for localization of the particles within organ structures. Although the particles do not have a plasmon peak, their absorbance in the near-infrared (NIR) is sufficient for PA excitation. PA imaging shows an increase of signal as particle concentrations increase, with changes in spectrum if particles aggregate. Fluorescence imaging using the particles’ native NIR emission shows agreement in general intensity in each organ, though quenching of emission can be seen at very high concentrations. Both of these imaging techniques are noninvasive and labor-saving alternatives to organ digestion and ICP-MS and may provide insight into cellular distribution of particles. The simple construct avoids the use of toxic semiconductor materials or dyes, relying upon the gold itself for both the fluorescence and PA signal. This provides a useful alternative to more complex approaches to multimodal imaging and one that is readily translatable to the clinic.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Wilson Poon ; Andrew Heinmiller ; Xuan Zhang and Jay L. Nadeau
"Determination of biodistribution of ultrasmall, near-infrared emitting gold nanoparticles by photoacoustic and fluorescence imaging", J. Biomed. Opt. 20(6), 066007 (Jun 23, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.066007


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.