Research Papers: Imaging

Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics

[+] Author Affiliations
Maria Gaertner, Christian Schnabel, Lars Kirsten, Edmund Koch

Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstraße 74, Dresden 01307, Germany

Kerstin Schirrmann, Ulrich Kertzscher

Charité - Universitätsmedizin Berlin, Labor für Biofluidmechanik, Augustenburger Platz 1, Berlin 13353, Germany

Sven Meissner

Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Fetscherstraße 74, Dresden 01307, Germany

EVONTA-Technology GmbH, Maria-Reiche-Straße 1, Dresden 01109, Germany

J. Biomed. Opt. 20(6), 066009 (Jun 25, 2015). doi:10.1117/1.JBO.20.6.066009
History: Received March 5, 2015; Accepted May 28, 2015
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Abstract.  Intravital microscopy (IVM) is a well-established imaging technique for real-time monitoring of microscale lung tissue dynamics. Although accepted as a gold standard in respiratory research, its characteristic image features are scarcely understood, especially when trying to determine the actual position of alveolar walls. To allow correct interpretation of these images with respect to the true geometry of the lung parenchyma, we analyzed IVM data of alveoli in a mouse model in comparison with simultaneously acquired optical coherence tomography images. Several IVM characteristics, such as double ring structures or disappearing alveoli in regions of liquid filling, could be identified and related to the position of alveoli relative to each other. Utilizing a ray tracing approach based on an idealized geometry of the mouse lung parenchyma, two major reflection processes could be attributed to the IVM image formation: partial reflection and total internal reflection between adjacent alveoli. Considering the origin of the reflexes, a model was developed to determine the true position of alveolar walls within IVM images. These results allow thorough understanding of IVM data and may serve as a basis for the correction of alveolar sizes for more accurate quantitative analysis within future studies of lung tissue dynamics.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Maria Gaertner ; Kerstin Schirrmann ; Christian Schnabel ; Sven Meissner ; Ulrich Kertzscher, et al.
"Toward a comprehensive interpretation of intravital microscopy images in studies of lung tissue dynamics", J. Biomed. Opt. 20(6), 066009 (Jun 25, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.6.066009


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