All the ablations were performed on ex vivo porcine eyes within five hours of procuring the eyes. The porcine eyes were stored in glucose solution (1L saline solution of Dulbecco's Modified Eagle Medium low glucose solution from PAA Laboratories GmbH) at room temperature (), during transportation and before ablation. Every eye was mounted on a holder and was recorded with a thermal camera during ablation. A customized AMARIS laser system (SCHWIND eye-tech-solutions, Kleinostheim, Germany) allowing for manipulation of ablation parameters was used for performing the ablations with only a single fluence. The treatment plane was calibrated to the corneal vertex with the help of a slit lamp from the laser system. The impact of system and ablation parameters such as local frequency, system repetition rate, pulse energy, OZ, and refractive correction were analyzed, with only one parameter varied at a time. The system and ablation specifications for each test setting are presented in Table 1. For most of the ablations, the eyes were de-epithelized manually prior to ablation (using an Amoils brush). However, to analyze the role of epithelium in temperature control, items 32 and 34 (in Table 1) were each ablated on porcine eyes with natural epithelium. Each item in the Table 1 was repeated in three different eyes to analyze repeatability; additionally, and in items 33 and 35 represent consecutive ablations performed in the same eye (one on top of the other after a time gap of ) to analyze the thermal response of the outer and comparatively deeper stromal layers. The ablations represented with and had identical ablation parameters. The items 33 () and 35 () were also repeated in three different eyes. Therefore, items 32 and 34 analyzed the corneal thermal response to transepithelial ablations, items 33 and 35 () analyzed the corneal thermal response to first stromal ablations (closer to the corneal surface) while items 33 and 35 () analyzed the corneal thermal response to second stromal ablations (starting at deeper layers within the same eye). The second stromal ablation was performed (nominal depth of the treated correction of with an OZ 6.3 mm) deeper than the first stromal layer. It must be noted that with the first stromal ablation ( and ), the nominal ablation depth of was achieved only in the center. In order to bring the periphery in level with the center (within the OZ) and achieve a flat stromal bed, before the second stromal ablation, the cornea was first ablated with a hyperopic profile (with no ablation at the center and ablation at the periphery). Subsequently, the second stromal ablation was performed (represented by and in Table 1) on a relatively flat stromal bed.