Special Section on Quantitative Phase Imaging in Biomedicine

Holographic fluorescence microscopy with incoherent digital holographic adaptive optics

[+] Author Affiliations
Changwon Jang, Jonghyun Kim, Seungjae Lee, Byoungho Lee

Seoul National University, School of Electrical Engineering, Gwanak-Gu Gwanakro 1, Seoul 151-744, Republic of Korea

David C. Clark, Myung K. Kim

University of South Florida, Department of Physics, ISAA6218, 4202 East Fowler Avenue, Tampa, Florida 33620, United States

J. Biomed. Opt. 20(11), 111204 (Jul 06, 2015). doi:10.1117/1.JBO.20.11.111204
History: Received March 16, 2015; Accepted June 8, 2015
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Abstract.  Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: self­interference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Changwon Jang ; Jonghyun Kim ; David C. Clark ; Seungjae Lee ; Byoungho Lee, et al.
"Holographic fluorescence microscopy with incoherent digital holographic adaptive optics", J. Biomed. Opt. 20(11), 111204 (Jul 06, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.11.111204


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