Research Papers: Imaging

Evaluation of light scattering and absorption properties of in vivo rat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem

[+] Author Affiliations
Sharmin Akter, Izumi Nishidate

Tokyo University of Agriculture and Technology, Graduate School of Bio-Application and Systems Engineering, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan

Satoshi Maejima, Akinari Hinoki, Suefumi Aosasa, Junji Yamamoto

National Defense Medical College, Department of Surgery, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

Satoko Kawauchi, Shunichi Sato

National Defense Medical College Research Institute, Division of Biomedical Information Sciences, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan

J. Biomed. Opt. 20(7), 076010 (Jul 27, 2015). doi:10.1117/1.JBO.20.7.076010
History: Received April 3, 2015; Accepted June 25, 2015
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Abstract.  Diffuse reflectance spectroscopy (DRS) has been extensively used for characterization of biological tissues as a noninvasive optical technique to evaluate the optical properties of tissue. We investigated a method for evaluating the reduced scattering coefficient μs, the absorption coefficient μa, the tissue oxygen saturation StO2, and the reduction of heme aa3 in cytochrome c oxidase CcO of in vivo liver tissue using a single-reflectance fiber probe with two source-collector geometries. We performed in vivo recordings of diffuse reflectance spectra for exposed rat liver during the ischemia–reperfusion induced by the hepatic portal (hepatic artery, portal vein, and bile duct) occlusion. The time courses of μa at 500, 530, 570, and 584 nm indicated the hemodynamic change in liver tissue as well as StO2. Significant increase in μa(605)/μa(620) during ischemia and after euthanasia induced by nitrogen breathing was observed, which indicates the reduction of heme aa3, representing a sign of mitochondrial energy failure. The time courses of μs at 500, 530, 570, and 584 nm were well correlated with those of μa, which also reflect the scattering by red blood cells. On the other hand, at 700 and 800 nm, a temporary increase in μs and an irreversible decrease in μs were observed during ischemia–reperfusion and after euthanasia induced by nitrogen breathing, respectively. The change in μs in the near-infrared wavelength region during ischemia is indicative of the morphological changes in the cellular and subcellular structures induced by the ischemia, whereas that after euthanasia implies the hepatocyte vacuolation. The results of the present study indicate the potential application of the current DRS system for evaluating the pathophysiological conditions of in vivo liver tissue.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Sharmin Akter ; Satoshi Maejima ; Satoko Kawauchi ; Shunichi Sato ; Akinari Hinoki, et al.
"Evaluation of light scattering and absorption properties of in vivo rat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem", J. Biomed. Opt. 20(7), 076010 (Jul 27, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.7.076010


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