Research Papers: Imaging

Confocal Raman microscopy to monitor extracellular matrix during dental pulp stem cells differentiation

[+] Author Affiliations
Hamideh Salehi, Pierre-Yves Collart-Dutilleul, Frédéric J. G. Cuisinier

Université de Montpellier, Laboratoire Bioingénierie et Nanoscience (LBN), EA 4203, Avenue du Professeur Jean Louis Viala, Montpellier 34193, France

Csilla Gergely

Université de Montpellier, Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS, Place Eugène Bataillon, Montpellier 34095, France

J. Biomed. Opt. 20(7), 076013 (Jul 27, 2015). doi:10.1117/1.JBO.20.7.076013
History: Received March 18, 2015; Accepted June 29, 2015
Text Size: A A A

Abstract.  Regenerative medicine brings promising applications for mesenchymal stem cells, such as dental pulp stem cells (DPSCs). Confocal Raman microscopy, a noninvasive technique, is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800 to 3000cm1 region (C-H stretching) and the 960cm1 peak (ν1PO43) were collected (to image cells and phosphate, respectively), and the ratio of two peaks 1660 over 1690cm1 (amide I bands) to measure the collagen cross-linking has been calculated. Raman spectra of DPSCs after 21 days differentiation reveal several phosphate peaks: ν1 (first stretching mode) at 960cm1, ν2 at 430cm1, and ν4 at 585cm1 and collagen cross-linking can also be calculated. Confocal Raman microscopy enables monitoring osteogenic differentiation in vitro and can be a credible tool for clinical stem cell based research.

Figures in this Article
© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Hamideh Salehi ; Pierre-Yves Collart-Dutilleul ; Csilla Gergely and Frédéric J. G. Cuisinier
"Confocal Raman microscopy to monitor extracellular matrix during dental pulp stem cells differentiation", J. Biomed. Opt. 20(7), 076013 (Jul 27, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.7.076013


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.