In preparation for the implementation of the device to observe a biospecimen using TPLSM, we introduced the device to a TPLSM illuminated by 950-nm-wavelength laser light and observed the fluorescent beads in order to confirm the correction effect of the device. The correction collar of the objective lens was adjusted to obtain the best performance at . As with single-photon excitation microscopy, the spatial resolution and the fluorescence signals of the images degraded in proportion to the depth where the beads were located [Figs. 5(a)–5(d), 5(g), and 5(h)]. At , the peak intensity dropped to 67% . Also, the and values degraded to from , and to from , respectively [Figs. 5(k) and 5(l)]. Furthermore, at , the peak intensity dropped to 45% . The value degraded to , and the value degraded to [Fig. 5(m)]. On the other hand, at and 0.6 mm, the device recovered 115% and 98% , respectively. The values at and 0.6 mm improved to 1.9 and , correspondingly, and the values to [Figs. 5(e), 5(f), 5(i), 5(j), 5(l), and 5(m)]; namely, the correction by the device increased the peak intensity by approximately 1.7 and 2.2 times, respectively, compared to the aberrated conditions. The FWHM values improved to the value almost equal to those at . At and 0.6 mm, the phase amplitudes generated by the device were 1550 and 3300 nm, respectively. In this way, we succeeded in improving the lateral and axial resolutions. Additionally, the fluorescence signal in deep regions was improved like in the case of single-photon excitation microscopy.