Research Papers: Imaging

Polarization dependence of aligned collagen tissues imaged with second harmonic generation microscopy

[+] Author Affiliations
Francisco J. Ávila, Oscar del Barco, Juan M. Bueno

Universidad de Murcia, Instituto Universitario de Investigación en Óptica y Nanofísica, Laboratorio de Óptica, Campus de Espinardo (Ed. 34), Murcia 30100, Spain

J. Biomed. Opt. 20(8), 086001 (Aug 11, 2015). doi:10.1117/1.JBO.20.8.086001
History: Received January 26, 2015; Accepted June 30, 2015
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Abstract.  A polarimetric second harmonic generation (SHG) microscope was used to analyze the dependence between polarization and SHG signal from collagen-based samples. A theoretical model was also developed to investigate the SHG intensity as a function of different polarization states for a set of quasiparallel fibers. Numerical simulations were compared to experimental SHG intensity values and a fairly good agreement was found. Linear polarized light produced periodical changes in the emitted SHG signal with a maximum of intensity corresponding to polarization parallel to the main orientation of the fibers, regardless the ratio of hyperpolarizabilities, ρ. A similar behavior was found for elliptical states located along a vertical meridian on the Poincaré sphere (i.e., null azimuth) although the modulation of the SHG signal was different. Our numerical calculations described a dramatic change in this regular trend when ρ changed from positive to negative values. Moreover, we provide an experimental method (based on the analysis of the modulation of the SHG signal) to determine the value of the ratio ρ and, consequently, to obtain information about the internal organization of the collagen fibers.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Francisco J. Ávila ; Oscar del Barco and Juan M. Bueno
"Polarization dependence of aligned collagen tissues imaged with second harmonic generation microscopy", J. Biomed. Opt. 20(8), 086001 (Aug 11, 2015). ; http://dx.doi.org/10.1117/1.JBO.20.8.086001


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